ABN327

Anti-SCRAPPER Antibody

from rabbit, purified by affinity chromatography

• Synonyme(s) : F-box, Leucine rich repeat protein 20, FBXL20, FBl2, FBL20
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Technique(s): immunohistochemistry: suitable; western blot: suitable
• Numéro d'accès UniProt: Q96IG2
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FBXL20(84961)

Description

Description générale

Members of the F-box protein family, such as SCRAPPER, are characterized by an approximately 40-amino acid F-box motif. SCRAPPER is selectively expressed in the brain, broadly expressed within the mouse CNS, and is abundant at the presynaptic membrane. It has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. SCRAPPER is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.

Immunogène

KLH-conjugated linear peptide corresponding to human SCRAPPER.

Application

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

This SCRAPPER antibody is validated for use in WB & IHC for the detection of the SCRAPPER protein.

Western Blot Analysis: 1 µg/mL from a representative lot detected SCRAPPER in 10 µg/mL of RAW264.7 cell lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected SCRAPPER in human midbrain tissue.

Qualité

Evaluated by Western Blot in human spleen tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SCRAPPER in 10 µg/mL of human spleen tissue lysate.

Description de la cible

~48 kDa observed. Uncharacterized bands may appear at ~52 kDa, ~58 kDa and ~70 kDa in some lysates.

Forme physique

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
Human spleen tissue lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable