ABE462
Anti-phospho ATM/ATR (Thr1989) Antibody
1.0 mg/mL, from rabbit
Synonyme(s) :
Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Produit purifié par
affinity chromatography
Espèces réactives
human
Concentration
1.0 mg/mL
Technique(s)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
phosphorylation (pThr1989)
Informations sur le gène
human ... ATM(472)
Description générale
The Ataxia-Telangiectasia and Rad3 related kinase (ATR) is a nuclear serine/threonine kinase that is part of the "first-response" system to DNA damage induced by a wide variety of factors including double-stranded breaks and replication stress. The localization of ATR to sites of DNA damage is aided by the Replication Protein A (RPA). Phospho - ATR (Ser428) targets a number of proteins including BRCA1, WRN, CHEK1, MCM2, and p53/TP53, and coordinates signaling pathways involved in DNA repair and apoptosis. ATR may also be involved in the phosphorylation of the histone H2A.X after replication stress, an early marker of DNA damage. However, ATR also mediates DNA replication and fork stability in normal cell cycles by activation of Chk1. Defective ATR contributes to Seckel syndrome type 1.
Immunogène
Linear peptide corresponding to human ATM/ATR (Thr1989).
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Nuclear Receptors
Nuclear Receptors
This Anti-phospho ATM/ATR (Thr1989) Antibody is validated for use in Western Blotting for the detection of phospho ATM/ATR (Thr1989).
Western Blotting Analysis: A representative lot detected ATM/ATR (Thr1989) in ATR TR cells (Nam, E.A, et al. (2011). JBC. 286(33):28707–28714).
Qualité
Evaluated by Western Blotting in 2mM hydroxyurea treated 293T cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ATM/ATR (Thr1989) in 10 µg of 2mM hydroxyurea treated 293T cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected ATM/ATR (Thr1989) in 10 µg of 2mM hydroxyurea treated 293T cell lysate.
Description de la cible
~300 kDa observed
Forme physique
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Hyoung Kim et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 23(12), 3097-3108 (2016-12-21)
Purpose: PARP inhibition (PARPi) has modest clinical activity in recurrent BRCA-mutant (BRCAMUT) high-grade serous ovarian cancers (HGSOC). We hypothesized that PARPi increases dependence on ATR/CHK1 such that combination PARPi with ATR/CHK1 blockade results in increased cell death and tumor regression.Experimental
Thr-1989 phosphorylation is a marker of active ataxia telangiectasia-mutated and Rad3-related (ATR) kinase.
Nam, EA; Zhao, R; Glick, GG; Bansbach, CE; Friedman, DB; Cortez, D
The Journal of Biological Chemistry null
Katarzyna B Leszczynska et al.
Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, 121(2), 232-238 (2016-11-15)
Esophageal cancer has a persistently low 5-year survival rate and has recently been classified as a cancer of unmet need by Cancer Research UK. Consequently, new approaches to therapy are urgently required. Here, we tested the hypothesis that an ATR
Yannick P Kok et al.
Oncogenesis, 9(10), 88-88 (2020-10-09)
Oncogene-induced replication stress, for instance as a result of Cyclin E1 overexpression, causes genomic instability and has been linked to tumorigenesis. To survive high levels of replication stress, tumors depend on pathways to deal with these DNA lesions, which represent
Haineng Xu et al.
Cell reports. Medicine, 2(9), 100394-100394 (2021-10-09)
CCNE1-amplified ovarian cancers (OVCAs) and endometrial cancers (EMCAs) are associated with platinum resistance and poor survival, representing a clinically unmet need. We hypothesized that dysregulated cell-cycle progression promoted by CCNE1 overexpression would lead to increased sensitivity to low-dose WEE1 inhibition
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