ABE1409

Anti-Lysine-Specific Demethylase 4C (JMJD2C)

from rabbit, purified by affinity chromatography

• Synonyme(s) : Gene amplified in squamous cell carcinoma 1 protein, GASC-1 protein, JmjC domain-containing histone demethylation protein 3C, Jumonji domain-containing protein 2C, Jmjd2C
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human, mouse
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_055876
• Numéro d'accès UniProt: Q9H3R0
• Conditions d'expédition: ambient
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... KDM4C(23081)

Description

Description générale

Lysine-specific demethylase 4C (UniProt: Q9H3R0; also known as EC: 1.14.11.-; Gene amplified in squamous cell carcinoma 1 protein, GASC-1 protein, JmjC domain-containing histone demethylation protein 3C, Jumonji domain-containing protein 2C, Jmjd2c) is encoded by the KDM4C (also known as GASC1, JHDM3C, JMJD2C, KIAA0780) gene (Gene ID: 23081) in human. Jmjd2c is an ubiquitously expressed histone demethylase that specifically demethylates Lys-9 and Lys-36 residues of histone H3. It is shown to demethylate only the trimethylated H3 and does not affect mono- and dimethylated residues. It does neither demethylate histone Lys-4 or Lys-27 of histone H3 nor the Lys-20 of histone H4. Human Jmjd2C contains one jumonji N domain (aa 16-58), a catalytic jumonji C domain (aa 144-310), two PHD zinc finger regions (aa 689-865), and two TUDOR methylated-histone binding domains (aa 877-991). Jmjd2c, along with its closely demethylase Jmjd2b, plays a vital role in self-renewal of embryonic stem cells (ESC) and in generation of induced pluripotent stem cells. Full-length Jmjd2b and Jmjd2c are shown to restore the normal ESC growth phenotype, ESC colony morphology, and also SSEA-1 expression. Jmjd2c is reported to assist polycomb repressive complex (PRC) in transcriptional repression. It is overexpressed in several esophageal squamous cell carcinomas.

Spécificité

This polyclonal antibody detects Lysine-specific demethylase 4C in multiple human and mouse cell types.

Immunogène

KLH-Conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human Lysine-specific demethylase 4C.

Application

Anti-Lysine-Specific Demethylase 4C (JMJD2C) Antibody, Cat. No. ABE1409, is a highly specific rabbit polyclonal antibody that targets Lysine-specific demethylase 4C and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 2 µg/mL from a representative lot detected Lysine-Specific Demethylase 4C (JMJD2C) in 10 µg of C2C12 and HEK293 nuclear extract.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Lysine-Specific Demethylase 4C (JMJD2C) in human tonsil, human cervical cancer, and human liver cancer tissues.

Qualité

Evaluated by Western Blotting in BALB/3T3 clone A31 cell nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected Lysine-Specific Demethylase 4C (JMJD2C) in 10 µg of BALB/3T3 clone A31 nuclear extract.

Description de la cible

~120 kDa observed; 119.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.