ABC953
Anti-AATF Antibody/Rb-binding protein Che-1
serum, from rabbit
Synonyme(s) :
Protein AATF, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
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Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
serum
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
human
Technique(s)
ChIP: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... AATF(26574)
Description générale
AATF/Rb-binding protein Che-1, also known as Protein Apoptosis-antagonizing transcription factor (Protein AATF), Rb-binding protein Che-1, and encoded by the gene name AATF/ CHE1/ DED/ HSPC277, is a member of the AATF family. Recent studies have indicated that AATF may function as a general inhibitor of the histone deacetylase HDAC1. AATF binds to the pocket region of RB1, and consequently it may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. In addition, AATF antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. AATF/Rb-binding protein Che-1 is expressed at high levels in brain, heart, kidney, placenta and thymus.
Immunogène
Epitope: POLR2J domain
Linear peptide corresponding to the POLR2J domain of human AATF/Rb-binding protein Che-1.
Application
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-AATF antibody is validated for use in WB, IC, IP, ChIP for the detection of AATF.
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in U2OS, T98G, LoVo, HT29, MCF7, HeLa, LIPARI, Jurkat, and U2OS cells were immunoprecipitated with anti-Rb (Fanciulli, M., et al. (2000). FASEB. 14:904-912).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in NIH 3T3 transfected cells, Synchronized NIH 3T3, HFF human fibroblasts, and HEK293 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, AT fibroblasts, NIH 3T3 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
Immunocytochemistry Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in Synchronized NIH 3T3, HFF human fibroblasts, HEK293, Rb transfected HEK293 cells, and Che-1 transfected NIH3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in NIH 3T3 transfected cells, Synchronized NIH 3T3, HFF human fibroblasts, and HEK293 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Western Blotting Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, AT fibroblasts, NIH 3T3 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
Immunocytochemistry Analysis: A representative lot detected AATF/Rb-binding protein Che-1 in HCT116, HCT15, human lymphoblastoid AHH1 cells, and CV1576 lymphoblastoid cells (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Sorino, C., et al. (2013). J. Biol. Chem. 288:23348-23357).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in NIH 3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in Synchronized NIH 3T3, HFF human fibroblasts, HEK293, Rb transfected HEK293 cells, and Che-1 transfected NIH3T3 (Bruno, T., et al. (2002). Cancer Cell. 2:387-399).
Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated AATF/Rb-binding protein Che-1 in HCT116 (Bruno, T., et al. (2006). Cancer Cell. 10:473-486).
Qualité
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected AATF/Rb-binding protein Che-1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected AATF/Rb-binding protein Che-1 in 10 µg of HeLa cell lysate.
Description de la cible
~70 kDa observed. The calculated molecular weight is 63 kDa, however AATF/Rb-binding protein Che-1 has been shown as a ~70 kDa band in western blots (Guo, G., et al. (2004). J. Biol. Chem. 279:4596-4603).
Forme physique
Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
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Zipper-interacting protein kinase is involved in regulation of ubiquitination of the androgen receptor, thereby contributing to dynamic transcription complex assembly.
Felten, A; Brinckmann, D; Landsberg, G; Scheidtmann, KH
Oncogene null
M Fanciulli et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(7), 904-912 (2000-04-27)
hRPB11 is a core subunit of RNA polymerase II (pol II) specifically down-regulated on doxorubicin (dox) treatment. Levels of this protein profoundly affect cell differentiation, cell proliferation, and tumorigenicity in vivo. Here we describe Che-1, a novel human protein that
Cristina Sorino et al.
The Journal of biological chemistry, 288(32), 23348-23357 (2013-06-27)
To combat threats posed by DNA damage, cells have evolved mechanisms, collectively termed DNA damage response (DDR). These mechanisms detect DNA lesions, signal their presence, and promote their repair. Centrosomes integrate G2/M checkpoint control and repair signals in response to
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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