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Key Documents

AB9304

Sigma-Aldrich

Anti-NAP-22 Antibody, complete sequence

Chemicon®, from rabbit

Synonyme(s) :

Neuronal acidic protein 22, CAP-23, BASP1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

rat

Fabricant/nom de marque

Chemicon®

Technique(s)

western blot: suitable

Numéro d'accès NCBI

NM_006317.3

Numéro d'accès UniProt

P80723

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

rat ... Basp1(64160)

Spécificité

Recognizes NAP-22 [Neuronal acidic protein 22, CAP-23, BASP1].

Immunogène

Epitope: Complete sequence
Recombinant full length rat NAP-22.

Application

Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 55 kDa NAP-22 protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 10%. Overnight incubation with the antibody is recommended.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Neuroregenerative Medicine
This Anti-NAP-22 Antibody, complete sequence is validated for use in WB for the detection of NAP-22.

Forme physique

Format: Purified
Semi-purified immunoglobulin. Liquid in TBS containing 0.05% sodium azide.

Stockage et stabilité

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Remarque sur l'analyse

Control
POSITIVE CONTROL: Neonatal rat brain extract. NAP-22 is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze NAP-22 content by immunoblot.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

T Laux et al.
The Journal of cell biology, 149(7), 1455-1472 (2000-06-28)
The dynamic properties of the cell cortex and its actin cytoskeleton determine important aspects of cell behavior and are a major target of cell regulation. GAP43, myristoylated alanine-rich C kinase substrate (MARCKS), and CAP23 (GMC) are locally abundant, plasmalemma-associated PKC
D Frey et al.
The Journal of cell biology, 149(7), 1443-1454 (2000-06-28)
CAP23 is a major cortical cytoskeleton-associated and calmodulin binding protein that is widely and abundantly expressed during development, maintained in selected brain structures in the adult, and reinduced during nerve regeneration. Overexpression of CAP23 in adult neurons of transgenic mice

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