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Merck
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Key Documents

AB748

Sigma-Aldrich

Anti-Collagen Type IV Antibody

Chemicon®, from rabbit

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

human

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
immunohistochemistry: suitable

Adéquation

not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... COL4A1(1282)

Spécificité

Antibody reacts with native and heat denatured human type IV collagen. Less than 10% cross reactivity with collagen types I, III, and V, and less than 1% with human collagen II. Antiserum was passed over immobilized human blood plasma proteins, and human collagen types I, III, and V. Human plasma proteins do not interfere with binding to collagen.

Immunogène

Purified human placental collagen type IV

Application

Anti-Collagen Type IV Antibody detects level of Collagen Type IV & has been published & validated for use in ELISA, IH.
ELISA on human collagen type IV: 1:3,000

Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40, fresh frozen or acetone fixed specimens only.

Dot and slot blots: 1:300.

Not recommended for use in Western blot, or for use on paraffin-embedded tissue sections.

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Forme physique

Affinity purified antibody from cross-absorbed antiserum. Liquid in PBS containing mannitol, dextran and no preservatives.
Format: Purified

Stockage et stabilité

Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze / thaw cycles.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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