AB5312

Anti-Growth Associated Protein 43 Antibody

serum, Chemicon^®

• Synonyme(s) : GAP-43, Neuromodulin, B-50
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: serum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: mouse, human, monkey, rat, feline, bovine
• Fabricant/nom de marque: Chemicon^®
• Technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Numéro d'accès NCBI: NM_002045.2
• Numéro d'accès UniProt: P17677
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GAP43(2596)

Description

Description générale

GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity.

Spécificité

AB5312 specifically recognizes GAP-43 regardless of the protein′s phosphorylation state. This antiserum has been refered to in the literature as CG-1.

Immunogène

Purified GAP-43 from feline brain (McIntosh and Parkinson, 1990).

Application

Immunocytochemistry: 1:100-1:400 for mouse primary neurons, NTera2 neurons.

Immuohistochemistry: 1:2,000 on coronal sections of adult visual cortex (McIntosh and Parkinson, 1990). AB5312 has been used to stain GAP-43 in 4% paraformaldehyde-fixed paraffin-embedded tissue sections of mouse olfactory bulb (Legrier, 2001).

Western blot: 1:500 - 1:1,000. Detects a single band at approximately 50 kDa on western blots of protein extracts from adult visual cortex (McIntosh and Parkinson, 1990).

Immunoprecipitation

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neuroregenerative Medicine

Signaling Neuroscience

This Anti-Growth Associated Protein 43 Antibody is validated for use in IC, IH, IH(P), IP, WB for the detection of Growth Associated Protein 43.

Description de la cible

23.6 kDa

Forme physique

Serum. Liquid with 0.02% sodium azide added as a preservative.

Unpurified

Stockage et stabilité

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
Rat dorsal root ganglion tissue that has been subjected to a spinal nerve ligation, cultured neurons

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable