AB16503
Anti-Apaf-1 Antibody, CT
Chemicon®, from rabbit
Synonyme(s) :
Apoptosis Protease-activating Factor 1
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
affinity purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
human, rat, mouse
Fabricant/nom de marque
Chemicon®
Technique(s)
western blot: suitable
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
wet ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... APAF1(317)
Spécificité
Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and was designated Apaf1 (for apoptosis protease-activating factor 1; Zou et al. 1997; Cecconi et al. 1998). Apaf1 binds to cytochrome C (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 which is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (Li et al. 1997). Apaf1 can also associate with caspase-4 and caspase-8 (Hu et al. 1998). Apaf1 is ubiquitously expressed in human tissues (Zou et al. 1997).
Immunogène
Epitope: C-terminus
Rabbit anti-Apaf1 (C-terminus) polyclonal antibody was raised against a peptide corresponding to amino acids 1158 to 1177 of human Apaf1 (Zou et al. 1997). The sequence of the immunogenic peptide differs from that of murine Apaf1 by one amino acid (Zou et al. 1997; Cecconi et al. 1998).
Application
Anti-Apaf-1 Antibody, C-terminus detects level of Apaf-1 & has been published & validated for use in WB.
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western blot: 1:500 to 1:2000.
Human heart tissue lysate can be used as positive control and a 130 kDa band should be detected.
Optimal working dilutions must be determined by end user.
Human heart tissue lysate can be used as positive control and a 130 kDa band should be detected.
Optimal working dilutions must be determined by end user.
Liaison
Replaces: 04-579
Forme physique
Format: Purified
Purified IgG by immunoaffinity chromatography. Liquid in PBS containing 0.02% sodium azide.
Stockage et stabilité
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Age-related alterations in expression of apoptosis regulatory proteins and heat shock proteins in rat skeletal muscle.
Linda Chung, Yuk-Chow Ng
Biochimica et Biophysica Acta null
Ignacio Vega-Naredo et al.
Methods in molecular biology (Clifton, N.J.), 2310, 161-178 (2021-06-08)
Mitochondria play a key role in cell death and its regulation. The permeabilization of the outer mitochondrial membrane, which is mainly controlled by proteins of the BCL-2 family, is a key event that can be directly induced by different signaling
Parco M Siu et al.
American journal of physiology. Cell physiology, 288(2), C338-C349 (2004-10-16)
The influence of aging on skeletal myocyte apoptosis is not well understood. In this study we examined apoptosis and apoptotic regulatory factor responses to muscle atrophy induced via limb unloading following loading-induced hypertrophy. Muscle hypertrophy was induced by attaching a
F Cecconi et al.
Cell, 94(6), 727-737 (1998-09-30)
The cytosolic protein APAF1, human homolog of C. elegans CED-4, participates in the CASPASE 9 (CASP9)-dependent activation of CASP3 in the general apoptotic pathway. We have generated by gene trap a null allele of the murine Apaf1. Homozygous mutants die
Parco M Siu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 18(10), 1150-1152 (2004-05-11)
The effect of exercise on apoptosis in postmitotic tissues is not known. In this study, we investigated the effect of regular moderate physical activity (i.e., exercise training) on the extent of apoptosis in rat skeletal and cardiac muscles. Adult Sprague
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