AB16501

Anti-AIF Antibody, internal domain

Chemicon^®, from rabbit

• Synonyme(s) : Apoptosis Inducing Factor
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Espèces réactives: human, mouse, rat
• Fabricant/nom de marque: Chemicon^®
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NM_004208.2; NM_145812.1; NM_145813.1
• Numéro d'accès UniProt: O95831
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... AIFM1(9131)

Description

Description générale

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami et al., 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al., 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (Susin et al., 1999).

Spécificité

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami & Kroemer 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al. 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (Susin et al. 1999).

Immunogène

Epitope: internal domain

Rabbit anti-AIF (Internal region) polyclonal antibody was raised against a peptide corresponding to amino acids 517 to 531 of human AIF (Susin et al. 1999). This sequence is identical to those of mouse and rat AIF (Susin et al. 1999).

Application

Anti-AIF Antibody, internal domain is an antibody against AIF for use in IH(P) & WB.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western blot: 0.25 to 1 μg/mL.

K562 cell lysate can be used as a positive control and a 67 kDa band should be detected.

Immunohistochemistry on formalin fixed, paraffin sections: 5-20 μg/ml, with antigen retrieval; see website protocol for details and photographs.

Optimal working dilutions must be determined by end user.

Description de la cible

67 kDa

Liaison

Replaces: 04-430

Forme physique

Ammonium sulfate precipitation and DEAE-cellulose chromatography

Format: Purified

Purified IgG in PBS containing 0.02% sodium azide.

Stockage et stabilité

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
Widely expressed

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable