AB15500

Anti-Calcium Transporter 1 Antibody

Chemicon^®, from rabbit

• Synonyme(s) : CaT-1, ECAC2
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 300
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: rat
• Fabricant/nom de marque: Chemicon^®
• Technique(s): ELISA: suitable; western blot: suitable
• Numéro d'accès NCBI: NM_018646.2
• Numéro d'accès UniProt: Q9H1D0
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: rat ... Slc7A1(25648)

Description

Description générale

CaT-1 (rat/mouse 727 aa; human 725 aa, chromosome 7q33-q34; ~85 kDa) is also known as ECAC2, CAT-like (CATL) or TRPV6 (transient receptor potential cation channel, subfamily V, member 6) contains a cytoplasmic N-terminus, 6 ankyrin repeat domains, 6 transmembrane domains, an extracellular pore region between TM5-6, and cytoplasmic C-terminus. CaT-1 shows 75% sequence identity to epithelial Ca++ channel (ECaC) protein from rabbit kidney. In rat, CaT-1 transcript (3-Kb) is expressed in duodenum, proximal jejunum cecum and colon but not in kidney; whereas ECaC in rabbit is expressed in apical cell membranes in distal part of nephron (kidney), duodenum, jejunum and placenta.

Spécificité

Calcium transporter 1 (CaT-1, ECAC2). The immunogen sequence has no significant homology with CaT-2 or other proteins.

Other species have not yet been tested. The immunogen sequence is 100% conserved in mouse, 88% in humans and 94% in Cat-1/htrp8A and htrp8b proteins.

Immunogène

Synthetic peptide near the C-terminus of rat CaT-1.

Application

Anti-Calcium Transporter 1 Antibody detects level of Calcium Transporter 1 & has been published & validated for use in ELISA & WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Western blot: 1-10 µg/mL using ECL. The expected size of CaT-1 is ~83 kDa. However, posttranslational modification may alter its size depending upon the species and cell type.
Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20 µg/mL
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.

Forme physique

ImmunoAffinity Purified

Liquid in PBS with 0.1% BSA.

Stockage et stabilité

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids