AB15332

Anti-Nicotinic Acetylcholine Receptor α7 Antibody

Chemicon^®, from rabbit

• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: rat, mouse, human
• Fabricant/nom de marque: Chemicon^®
• Technique(s): western blot: suitable
• Numéro d'accès NCBI: NM_000746.3
• Numéro d'accès UniProt: P36544
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: mouse ... Chrna7(11441)

Description

Spécificité

This rabbit polyclonal antibody specifically detects Nicotinic Receptor 7. It targets an epitope with 28 amino acids from the C-terminal half.

Immunogène

KLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal half of human Nicotinic Receptor 7.

Application

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.Western Blotting Analysis: A 1:500 dilution of this antibody detected Nicotinic Acetylcholine Receptor 7 in Rat brain tissue lysate.

Description de la cible

Neuronal acetylcholine receptor subunit alpha-7 (UniProt: P36544; also known as -Nicotinic Receptor Alpha 7, Nicotinic AChR alpha7) is encoded by the CHRNA7 (also known as NACHRA7) gene (Gene ID: 1139) in human. Nicotinic acetylcholine receptors are cation-selective, ligand-gated ion channels that mediate fast neurotransmission. They are distributed at the post-synaptic membrane of muscle cells. A similar neuronal-type ACh receptor is also found in the central nervous system and is involved in a wide range of physiological and pathological functions. They are assembled either as homo-pentamers of 7, 8, and 9 or hetero-pentamers of 2 6 in combination with β2 β4 or 9 with 10 subunits. They regulate the flow of sodium, potassium, and calcium ions across the cell membrane. The binding of ligands to these receptors triggers a tertiary conformational transition of neuronal AChRs among functionally distinct resting, open, and desensitized states. Nicotinic AChR 7 is a multi-pass, homopentameric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-230), four transmembrane domains (aa 231-255; 262-280; 296-317; and 470-490), and one cytoplasmic domain (aa 318-469). (Ref.: Ho, TN., et al. (2020). Front. Neurosci. 14; 609005; Unwin, N. (2013). Q. Rev. Biophys. 46(4); 283-322).

Liaison

Replaces: AB5637

Forme physique

Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.

Stockage et stabilité

Recommended storage: +2°C to +8°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable