565764

γ-Secretase Substrate, Fluorogenic

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase.

• Synonyme(s) : γ-Secretase Substrate, Fluorogenic, NMA-GGVVIATVK(DNP)- DR DR DR-NH₂
• Formule empirique (notation de Hill): C₇₀H₁₁₆N₂₆O₁₈
• Poids moléculaire : 1609.83
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic); protect from light
• Couleur: yellow
• Solubilité: DMSO: 5 mg/mL; 5% acetic acid: soluble
• Conditions d'expédition: ambient
• Température de stockage: −20°C

Description

Description générale

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ₄₀-, Aβ₄₂-, and Aβ₄₃-generating cleavage sites results in enhanced fluorescence.

An internally quenched fluorogenic peptide substrate containing the C-terminal β-APP (amyloid β-peptide precursor protein) amino acid sequence that is cleaved by γ-secretase. Shown to be sensitive and useful for assaying γ-secretase activity. The proteolysis at the Aβ₄₀-, Aβ₄₂-, and Aβ₄₃-generating cleavage sites results in enhanced fluorescence. Excitation max.: ~355 nm; emission max.: ~440 nm.

Actions biochimiques/physiologiques

Cell permeable: no

Product does not compete with ATP.

Reversible: no

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Séquence

NMA-Gly-Gly-Val-Val-Ile-Ala-Thr-Val-Lys(DNP)-D-Arg-D-Arg-D-Arg-NH₂

Reconstitution

Following reconstitution, aliquot, flush with a stream of inert gas, and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Farmery, M.R., et al. 2003. J. Biol. Chem.278, 24277.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable