551826

QUIN 2, Tetrapotassium Salt

Binding of Ca²⁺ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield.

• Synonyme(s) : QUIN 2, Tetrapotassium Salt, 2-{[2- bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8- bis(carboxymethyl)aminoquinoline, 4K, 2-{[2-bis(Carboxymethyl)amino-5-methylphenoxy]methyl}-6-methoxy-8-bis(carboxymethyl)aminoquinoline, 4K
• Formule empirique (notation de Hill): C₂₆H₂₃N₃O₁₀ · 4K
• Numéro CAS: 73630-23-6
• Poids moléculaire : 693.87
• Numéro MDL: MFCD00065487
• Code UNSPSC : 12352302
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: yellow
• Solubilité: water: 10 mg/mL
• Fluorescence: λ_ex 332 nm (high); λ_ex 354 nm (low); λ_em 505 nm (high); λ_em 510 nm (low)
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C26H27N3O10.4K/c1-15-3-6-19(28(10-22(30)31)11-23(32)33)21(7-15)39-14-17-5-4-16-8-18(38-2)9-20(26(16)27-17)29(12-24(34)35)13-25(36)37;;;;/h3-9H,10-14H2,1-2H3,(H,30,31)(H,32,33)(H,34,35)(H,36,37);;;;/q;4*+1/p-4
• Clé InChI: XAGUNWDMROKIFJ-UHFFFAOYSA-J

Description

Description générale

Binding of Ca²⁺ leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells.

Binding of Ca²⁺ to QUIN 2 leads to a major shift in UV absorption spectrum and a twenty-fold enhancement in fluorescence quantum yield. TPEN (Cat. No. 616394) can be used in conjunction with QUIN 2 to reduce heavy-metal quenching of QUIN 2 fluorescence in cells. Spectral properties in the presence of low calcium concentrations (ex. 354 nm, em. 510 nm) differ from those during high calcium conditions (ex. 332 nm, em. 505 nm).

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Calcium Probes

Product does not compete with ATP.

Reversible: no

Avertissement

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) under an inert gas, pH >10. Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Komada, H., et al. 1989. Cell Struct. Funct. 14, 141.
Vijayaraghavan, S. and Hoskins, D. 1989. Cell Calcium10, 241.
Arslan, P., et al. 1985. J. Biol. Chem.260, 2719.
Bryant, D.T. 1985. Biochem. J.226, 613.
Grynkiewicz, G., et al. 1985. J. Biol. Chem.260, 3440.
Ashley R.H., et al. 1984. Biochem. J.219, 149.
Tsien, R.Y., et al. 1982. J. Cell Biol.94, 325.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable