444235-M

MMP-13 Substrate, Fluorogenic

A quenched fluorescence substrate for MMP-13 (collagenase-3; k_cat/Km = 1.09 x 10⁶ M⁻¹s⁻¹).

• Synonyme(s) : MMP-13 Substrate, Fluorogenic, Collagenase-3 Substrate, Fluorogenic
• Formule empirique (notation de Hill): C₅₁H₆₅N₁₃O₁₅
• Poids moléculaire : 1100.14
• Code UNSPSC : 12352200

Propriétés

• Niveau de qualité: 100
• Pureté: ≥98% (HPLC)
• Forme: lyophilized solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated; protect from light
• Couleur: yellow
• Solubilité: DMSO: 5 mg/mL
• Fluorescence: λ_ex ~325 nm; λ_em ~393 nm
• Température de stockage: −20°C

Description

Description générale

A quenched fluorescence substrate for MMP-13 (collagenase-3; k_cat/K_m = 1.09 x 10⁶ M^-1s^-1). Less efficient as a substrate for MMP-1 (fibroblast collagenase) or MMP-8 (neutrophil collagenase).

A quenched fluorescence substrate for MMP-13 (collagenase-3; k_cat/K_m = 1.09 x 10⁶ M^-1s^-1; excitation maximum: ~325 nm; emission maximum: ~393 nm). Less efficient as a substrate for MMP-1 (fibroblast collagenase) or MMP-8 (neutrophil collagenase).

Actions biochimiques/physiologiques

Primary Target
A quenched fluorescence substrate for MMP-13 (collagenase-3)

kcat/Km = 1.09 x 106 M⁻¹ sec⁻¹ for MMP-13 (collagenase-3)

Avertissement

Toxicity: Standard Handling (A)

Séquence

MCA-Pro-Cha-Gly-Nva-His-Ala-Dpa-NH₂ [Cha = L-cyclohexylalanine; Dpa = 3-(2,4-dinitrophenyl)-L-2,3-diaminopropionyl; Nva = L-norvaline]

Forme physique

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Autres remarques

Knauper, V., et al. 1996. J. Biol. Chem. 271, 1544.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable