444231

MMP-9, Monomer, Human Neutrophil

• Synonyme(s) : Matrix Metalloproteinase 9, Gelatinase B, 92 kDa Type IV Collagenase
• Numéro de classification (Commission des enzymes): 3.4.24.35 (BRENDA, IUBMB)
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Pureté: ≥90% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: liquid
• Activité spécifique: ≥1000 mU/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Activité étrangère: other MMP activity, none detected
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

Monomeric, native MMP-9 from stimulated human neutrophils. Matrix metalloproteinases (MMP) are members of a family of at least 20 zinc-dependent endoproteinases that function extracellularly at neutral pH. The enzymes are synthesized as latent or inactive zymogens that contain a regulatory propeptide. This propeptide confers latency by binding to the Zn²⁺ molecule present in the active site. MMP activation can be achieved through autoactivation, via cleavage by other MMPs or proteases, such as furin, urokinase, plasmin, and trypsin, or by treatment with organomercurial comounds. This preparation is highly purified native MMP-9 from stimulated human neutrophils. Requires activation prior to use.

Monomeric, native MMP-9 from stimulated human neutrophils. Requires activation prior to use.

Note: 1 mU = 1 milliunit.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.

Forme physique

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Notes préparatoires

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Reynolds, M. A., et al. 2003. Clin. Chem.49, 1733.
Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.
Marcy, A.I., et al. 1991. Biochemistry30, 6476.
Stricklin, et al. 1983. Biochemistry22, 61.

Informations légales

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable