420320

KT5720

≥95% (HPLC), solid, protein kinase A inhibitor, Calbiochem^®

• Synonyme(s) : KT5720, PKA Inhibitor VII
• Formule empirique (notation de Hill): C₃₂H₃₁N₃O₅
• Numéro CAS: 108068-98-0
• Poids moléculaire : 537.61
• Numéro MDL: MFCD00132118
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.77

Propriétés

• product name: KT5720, A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (K_i = 56 nM) that is prepared by a chemical modification of K-252a.
• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: white
• Solubilité: DMSO: 1 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: −20°C
• InChI: 1S/C32H31N3O5/c1-3-4-5-10-15-39-30(37)32(38)16-23-34-21-13-8-6-11-18(21)25-26-20(17-33-29(26)36)24-19-12-7-9-14-22(19)35(28(24)27(25)34)31(32,2)40-23/h6-9,11-14,23,38H,3-5,10,15-17H2,1-2H3,(H,33,36)/t23-,31+,32+/m0/s1
• Clé InChI: ZHEHVZXPFVXKEY-RUAOOFDTSA-N

Description

Description générale

A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (K_i = 56 nM) that is prepared by a chemical modification of K-252a. Does not significantly affect the activities of PKC, PKG, and MLCK. Inhibits axon branching in cultured neurons. A 2 mM (50 µg/47 µl) solution of KT 5720 (Cat. No. 420323) in DMSO is also available.

Prepared by a chemical modification of K-252a. A potent, specific, cell-permeable, reversible and ATP-competitive inhibitor of protein kinase A (K_i = 56 nM). Does not significantly affect the activity of PKC, PKG, or MLCK. Inhibits axon branching in cultured neurons.

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
PKA

Product competes with ATP.

Reversible: yes

Target K_i: 56 nM for protein kinase A

Avertissement

Toxicity: Standard Handling (A)

Notes préparatoires

Further dilute with aqueous buffers just prior to use.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Simpson, C.S., and Morris, B.J. 1995. Neuroscience 68, 97.
Cabell, L., and Audesirk, G. 1993. Int. J. Dev. Neurosci.11, 357.
Gadbois, D.M., et al. 1992. Proc. Natl. Acad. Sci. USA89, 8626.
Kase, H., et al. 1987. Biochem. Biophys. Res. Commun.142, 436.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable