368067-M

Granzyme B Substrate VIII, Colorimetric

A colorimetric substrate for granzyme B (kcat/Km = 6.6 x 10⁴ M⁻¹s⁻¹).

• Synonyme(s) : Granzyme B Substrate VIII, Colorimetric, Ac-IEPD- pNA
• Formule empirique (notation de Hill): C₂₈H₃₈N₆O₁₁
• Poids moléculaire : 634.63
• Code UNSPSC : 12352200
• ID de substance PubChem : 2826717

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: lyophilized solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; protect from light
• Couleur: white
• Solubilité: DMSO: 5 mg/mL
• Température de stockage: −20°C
• Chaîne SMILES: CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)N1CCCC1C(=O)NC(CC(=O)O)C(=O)NC2=CC=C(C=C2)[N+](=O)[O-])NC(=O)C

Description

Description générale

A colorimetric substrate for granzyme B (k_cat/K_m = 6.6 x 10⁴ M^-1s^-1). Cleavage of pNA is monitored colorimetrically at ~405 nm (ε = 9,160 M^-1cm^-1).

A colorimetric substrate for granzyme B (k_cat/K_m = 66,600 M_-1s_-1). Cleavage of pNA is monitored as an increase in absorbance at ~405 nm (ε = 9,160 M_-1cm_-1).

Actions biochimiques/physiologiques

Primary Target
Colorimetric substrate for granzyme B

kcat/Km = 6.6 x 104 M⁻¹ sec⁻¹ as substrate for granzyme B

Avertissement

Toxicity: Standard Handling (A)

Séquence

Ac-Ile-Glu-Pro-Asp-pNA

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Autres remarques

Harris, J.L., et al. 1998. J. Biol. Chem. 273, 27364.
Thornberry, N.A., et al. 1997. J. Biol. Chem. 272, 17907.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable