SRE0021

Granzyme B human

recombinant, expressed in insect cells, ≥90% (SDS-PAGE)

• Synonyme(s) : Granzyme B human, Cytotoxic T-lymphocyte proteinase 2, Granzyme-2, GranzymeB, Lymphocyte protease, SECT, T-cell serine protease 1-3E
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Niveau de qualité: 200
• Produit recombinant: expressed in insect cells
• Pureté: ≥90% (SDS-PAGE)
• Forme: lyophilized powder
• Puissance: >0.004 units/mg
• Activité spécifique: ≥40 units/μg
• Conditionnement: pkg of 5 μg
• Numéro d'accès UniProt: P10144
• Conditions d'expédition: wet ice
• Température de stockage: −70°C
• Informations sur le gène: human ... GZMB(3002)

Description

Description générale

Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.

Actions biochimiques/physiologiques

Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.

Définition de l'unité

One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl₂.

Forme physique

Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable