322327

Diphtheria Toxin, CRM Mutant

Diphtheria Toxin, CRM197 Mutant, CAS 92092-36-9, is a nontoxic material with a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity.

• Synonyme(s) : Diphtheria Toxin, CRM Mutant
• Numéro CAS: 92092-36-9
• Code UNSPSC : 12352202

Propriétés

• Niveau de qualité: 100
• Forme: solid
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

CRM₁₉₇ is a nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity. Does not catalyze the ADP ribosylation of EF-2 factor. Immunologically, the CRM₁₉₇ protein is identical to the wild-type toxin.

Cross-reactive material of diphtheria toxin (CRM₁₉₇). A nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A that results in complete loss of enzymatic activity. Inhibits smooth muscle cell proliferation in a dose-dependent manner. Immunologically, the CRM₁₉₇ is identical to the wild-type toxin and is useful as a carrier protein of conjugate vaccines.

Actions biochimiques/physiologiques

Cell permeable: no

Primary Target
Mutant with loss of enzymatic activity to catalyze the ADP ribosylation of EF-2 factor

Reversible: no

Avertissement

Toxicity: Standard Handling (A)

Forme physique

Lyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.

Please refer to vial label for lot-specific reconstitution volume.

Remarque sur l'analyse

One major band by SDS-PAGE

Autres remarques

Kusano, I., et al. 2001. Cell Struct. Funct.26, 279.
Kaefer, M., et al. 2000. J. Urol.163, 580.
Gupta, R.K., et al. 1997. Vaccine15, 1341.
Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol.251, 175.
Giannini, G., et al. 1984. Nucleic Acids Res.12, 4063.
Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable