16-222

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate

clone 3H10, Upstate^®, from mouse

• Synonyme(s) : H3 histone, family 3A, H3S10P, Histone H3 (phospho S10), H3 histone, family 3A, H3 histone, family 3B, H3 histone, family 3B (H3.3B)
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Conjugué: FITC conjugate
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 3H10, monoclonal
• Espèces réactives: human
• Fabricant/nom de marque: Upstate^®
• Technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• Isotype: IgG
• Numéro d'accès NCBI: NP_003484
• Numéro d'accès UniProt: Q16695
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer10)
• Informations sur le gène: human ... HIST1H3F(8968)

Description

Description générale

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Spécificité

Broad species cross-reactivity is expected.

Recognizes histone H3 phosphorylated at Ser10, MW 17 kDa.

Immunogène

A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.

Epitope: Ser10

Application

Anti-Phospho-Histone H3 (Ser10) Antibody, clone 3H10, FITC Conjugate is a Mouse Monoclonal for detection of Histone H3 phosphorylated at serine 10. This mAb, also known as H3S10p,is labeled with Fluorescein isothiocyanate (FITC), published in peer reviewed journals & validated in WB, ICC & IF.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Western Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.

Qualité

Immunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4μg/mL of this antibody.

Description de la cible

17 kDa

Forme physique

Protein G Purified

Purified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.

Stockage et stabilité

Stable for 1 year at from date of receipt.

Remarque sur l'analyse

Control
Mitotic HeLa cells (IF).

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2