1.51450

Chromolith^® RP-18 endcapped (monolithic) HPLC Columns

L × I.D.50 mm x 4.6 mm HPLC Column

• Synonyme(s) : Chromolith^® SpeedROD RP-18 endcapped 50-4.6 HPLC column
• Code UNSPSC : 41115709
• Nomenclature NACRES : SB.52

Propriétés

• product name: Colonne Chromolith^® RP-18 Endcapped., L × I.D.50 mm x 4.6 mm HPLC column
• Matériaux: PEEK column
• Niveau de qualité: 100
• Agence: suitable for USP L1
• Gamme de produits: Chromolith^®
• Caractéristiques: endcapped
• Conditionnement: 1 ea of
• Ampleur du marquage: 18% Carbon loading
• Paramètres: 200 bar pressure; 45 °C max. temp.
• Technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• Colonne, L × D.I.: 5 cm × 4.6 mm
• Matrice: monolithic silica
• Groupe de la matrice active: C18 bonding phase
• Dimension de pores: 13 nm mesoporosity; 2 μm macroporosity
• operating pH range: 2-7.5
• Technique de séparation: reversed phase
• Température de stockage: no temp limit

Description

Description générale

Chromolith^® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^® SpeedROD RP-18 endcapped columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith^® SpeedROD RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith^® SpeedROD RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for fast separations on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith^® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
Or
Chromolith^® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451]
Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]
The Chromolith^® column coupler can be used to significantly increase the separation efficiency of Chromolith^® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Need more efficiency?
Chromolith^® HighResolution RP-18 endcapped 50-4.6 HPLC columns [1.52021] provide a column efficiency exceeding 140,000 plates/meter.

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Application

• Simultaneous determination of eight arginine-related metabolites in cellular extracts using liquid chromatography-tandem mass spectrometry.: This study explores the usage of the Chromolith^® RP-18 endcapped column for precise metabolic profiling, showcasing its effectiveness in analyzing arginine-related metabolites with high resolution and throughput (Amatya et al., 2020).


• Rapid Determination of 30 Polyphenols in Tongmai Formula, a Combination of Puerariae Lobatae Radix, Salviae Miltiorrhizae Radix et Rhizoma, and Chuanxiong Rhizoma, via Liquid Chromatography-Tandem Mass Spectrometry.: The use of Chromolith^® RP-18 endcapped allowed for the fast and efficient separation of polyphenols in complex herbal formulations, illustrating its capabilities in herbal chemistry applications (Wang et al., 2017).


• Simultaneous Liquid Chromatographic Determination of Ebastine with Two Sympathomimetic Drugs Using a Monolithic Column.: This research highlights the Chromolith^® RP-18 endcapped column′s ability to simultaneously analyze multiple compounds, thereby providing a method that improves the efficiency of pharmaceutical analyses (Ibrahim and Wahba, 2017).

Remarque sur l'analyse

Carbon content: 17.0 - 18.6 %
Pressure: ≤ 15 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.6 - 4.6
Tailing (USP): 0.90 - 1.55 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35

Informations légales

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany