1.25090

Résine Fractogel^® EMD SO₃ ⁻ (M)

RoboColumn Fractogel^® SO3 (M), volume 0.2 mL, prepacked column

• Synonyme(s) : RoboColumn Fractogel^® SO3 (M), 0.2 ml
• Code UNSPSC : 41115711

Propriétés

• ligand: (Sulfoisobutyl)
• Matériaux: polypropylene housing
• Gamme de produits: Fractogel^®
• Forme: prepacked column
• Paramètres: ≤8 bar pressure; ~200 cm/hr max. flow rate
• column I.D. × L: 5 mm × 10 mm
• Volume: 0.2 mL
• Groupe de la matrice active: Methacrylate
• Taille moyenne des particules: 40-90 μm
• Capacité: ~130 mL, resin binding capacity
• Application(s): mAb; recombinant protein; vaccine development
• Technique de séparation: cation exchange
• Température de stockage: room temp

Description

Description générale

5 x 10 mm

Caractéristiques et avantages

RoboColumn Fractogel^® SO3 (M), 0.2 ml enables:

• Increased binding strength at low pH
• High aggregate removal efficiency combined with high dynamic binding capacity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (M) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Informations légales

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany