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Key Documents

07-452

Sigma-Aldrich

Anti-dimethyl-Histone H3 (Lys27) Antibody

Upstate®, from rabbit

Synonyme(s) :

H3K27me2, Histone H3 (di methyl K27)

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

human

Fabricant/nom de marque

Upstate®

Technique(s)

ChIP: suitable (ChIP-seq)
cell based assay: suitable
dot blot: suitable
multiplexing: suitable
western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

NM_003493

Numéro d'accès UniProt

Q16695

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

dimethylation (Lys27)

Informations sur le gène

human ... H3C1(8350)

Description générale

Anti-dimethyl-Histone H3 (Lys27)

Spécificité

Broad species cross-reactivity expected, based on sequence homology
Recognizes dimethylated histone H3 (Lys27).

Immunogène

KLH-conjugated, synthetic 2X-branched peptide containing the sequence ...ARme2KSA... in which me2K corresponds to dimethyl-lysine at residue 27 of human histone H3

Application

Additional Referenced Research Applications:
Dot Blot, Immunocytochemistry (cells)
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-dimethyl-Histone H3 (Lys27) Antibody (rabbit polyclonal antibody) validated in Mplex, PIA, WB, ChIP-seq, DB to detect dimethyl-Histone H3 (Lys27) also known as H3K27me2, Histone H3 (di methyl K27).

Qualité

Routinely evaluated by immunoblot on acid extracted proteins from HeLa cells.

Description de la cible

17 kDa

Forme physique

Affinity purified IgG in 0.1M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% NaN3.
Peptide affinity purified

Stockage et stabilité

Stable for 2 years at 2-8°C from date of shipment.

Remarque sur l'analyse

Control
HeLa whole cell lysate, U2OS cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 polycomb group complexes.
Silva, Jose, et al.
Developmental Cell, 4, 481-495 (2003)
Epigenetic regulation of dendritic cell-derived interleukin-12 facilitates immunosuppression after a severe innate immune response.
Wen, H; Dou, Y; Hogaboam, CM; Kunkel, SL
Blood null
Reciprocal patterns of methylation of H3K36 and H3K27 on proximal vs. distal IgVH genes are modulated by IL-7 and Pax5.
Xu, CR; Schaffer, L; Head, SR; Feeney, AJ
Proceedings of the National Academy of Sciences of the USA null
Mathieu Lupien et al.
Cell, 132(6), 958-970 (2008-03-25)
Complex organisms require tissue-specific transcriptional programs, yet little is known about how these are established. The transcription factor FoxA1 is thought to contribute to gene regulation through its ability to act as a pioneer factor binding to nucleosomal DNA. Through
Pedro Crevillén et al.
Nature, 515(7528), 587-590 (2014-09-16)
The reprogramming of epigenetic states in gametes and embryos is essential for correct development in plants and mammals. In plants, the germ line arises from somatic tissues of the flower, necessitating the erasure of chromatin modifications that have accumulated at
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