07-2119

Anti-phospho-RalA Antibody (Ser194)

from rabbit, purified by affinity chromatography

• Synonyme(s) : Ras-related protein Ral-A
• Code UNSPSC : 12352203
• eCl@ss : 32160702

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: human
• Réactivité de l'espèce (prédite par homologie): rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• Technique(s): inhibition assay: suitable (peptide); western blot: suitable
• Numéro d'accès NCBI: NP_005393
• Numéro d'accès UniProt: P11233
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: phosphorylation (pSer194)
• Informations sur le gène: human ... RALA(5898)

Description

Description générale

RalA and RalB are Ras-family small monomeric GTPases that can be activated through a Ras-dependent mechanism. The two are highly homologous (89%). Ral proteins are proximal effectors of oncogenic Ras and their activity is needed to support tumorigenesis in certain cancers. Mutants of Ras that are defective in activating all known Ras effectors except Ral GEF are still able to induce invasive phenotypes in transfected cells, suggesting a role for Ral in these processes. RalA is required for anchorage-independent proliferation and tumorigeneisis by driving exocyst assembly and polarized exocytosis in epithelial cells.

Spécificité

This antibody recognizes RalA phosphorylated at Ser194.

Immunogène

KLH-conjugated linear peptide corresponding to human RalA phosphorylated at Ser194.

Application

Detect the phospho-RalA (Ser194) protein using this Anti-phospho-RalA (Ser194) validated for use in WB & Peptide Inhibition Assay.

Western Blot Analysis: A representative lot detected phospho-RalA (Ser194) in certain primary and metastatic cell lysates, and in certain unmatched tumor tissue lysate samples.

Qualité

Evaluated by Western Blot in PANC10.05 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected phospho-RalA in PANC10.05 cell lysate. Peptide inhibition was observed with the addition of phospho peptides but not with the addition of non-phospho peptides.

Description de la cible

~25 kDa observed. Uncharacterized bands may be observed at ~20 kDa, 100 kDa, and 130-170 kDa in some cell lysates.

Remarque sur l'analyse

Control
PANC10.05 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable