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Merck
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Key Documents

05-321MG

Sigma-Aldrich

Anti-Phosphotyrosine Antibody, clone 4G10®

clone 4G10®, Upstate®, from mouse

Synonyme(s) :

Clone 4G10 Anti-Phosphotyrosine, Phosphotyrosine Detection Antibody

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

4G10®, monoclonal

Réactivité de l'espèce (prédite par homologie)

all

Fabricant/nom de marque

Upstate®

Technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG2bκ

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

phosphorylation (pTyr)

Application

Anti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.

Qualité

Routinely evaluated on EGF-treated human A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).

Description de la cible

Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.

Forme physique

Format: Purified
Purified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC.
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10 (FOX-NY [NS-1 derivative] myeloma x spleen cells).

Remarque sur l'analyse

Control
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

4G10 is a registered trademark of Upstate Group, Inc.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Réf. du produit
Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Hoang Thanh Chi et al.
Biomedical reports, 19(1), 47-47 (2023-06-29)
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Hui Guo et al.
Acta pharmacologica Sinica, 39(3), 425-437 (2017-11-10)
STAT1 and STAT3 are two important members of the STAT (signal transducers and activators of transcription) protein family and play opposing roles in regulating cancer cell growth. Suppressing STAT3 and/or enhancing STAT1 signaling are considered to be attractive anticancer strategies.
Zhi Liu et al.
The Journal of biological chemistry, 290(12), 7535-7562 (2015-01-15)
Producing pure and well behaved bispecific antibodies (bsAbs) on a large scale for preclinical and clinical testing is a challenging task. Here, we describe a new strategy for making monovalent bispecific heterodimeric IgG antibodies in mammalian cells. We applied an
Shaobo Wang et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(1), 137-148 (2017-11-16)
Reelin controls neuronal migration and layer formation. Previous studies in reeler mice deficient in Reelin focused on the result of the developmental process in fixed tissue sections. It has remained unclear whether Reelin affects the migratory process, migration directionality, or

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