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Merck
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Key Documents

05-175

Sigma-Aldrich

Anti-Myb Antibody, clone 1-1

clone 1-1, Upstate®, from mouse

Synonyme(s) :

Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA), v-myb avian myeloblastosis viral oncogene homolog, v-myb avian myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral oncogene homolog, v-myb myeloblastosis viral on

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

1-1, monoclonal

Espèces réactives

mouse, human

Fabricant/nom de marque

Upstate®

Technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG2aκ

Numéro d'accès NCBI

NM_005375.2

Numéro d'accès UniProt

P10242

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MYB(4602)

Description générale

Myb is a transcriptional activator. It is a DNA-binding protein that specifically recognizes the sequence 5′-YAAC[GT]G-3′. Myb plays an important role in the control of proliferation and differentiation of hematopoietic progenitor cells.

Spécificité

This antibody recognizes Myb, Mr 80 kDa, containing C-terminal amino acids from 401-636 (Salomoni, P., 1997).

Immunogène

Recombinant murine Myb purified by SDS-PAGE (Nicot, C., 2001) Clone 1-1.

Application

Anti-Myb Antibody, clone 1-1 is a Mouse Monoclonal Antibody for detection of Myb also known as Avian myeloblastosis viral (v-myb) oncogene homolog 2 c-myb protein (140 AA) & has been validated in IHC, IP & WB.
Immunoprecipitation:
4 μg of previous lot immunoprecipitated Myb from Jurkat cell lysate.

Immunohistochemistry:
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

Qualité

Routinely evaluated on RIPA lysate from Jurkat cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected Myb in 20 μg of RIPA lysate from Jurkat cells.

Description de la cible

80 kDa

Forme physique

Ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography.
Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide. Frozen solution.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Remarque sur l'analyse

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Marc R Mansour et al.
The Journal of experimental medicine, 210(8), 1545-1557 (2013-07-17)
The oncogenic transcription factor TAL1/SCL is aberrantly expressed in 60% of cases of human T cell acute lymphoblastic leukemia (T-ALL) and initiates T-ALL in mouse models. By performing global microRNA (miRNA) expression profiling after depletion of TAL1, together with genome-wide
Zhenglong Wu et al.
Journal of experimental & clinical cancer research : CR, 32, 98-98 (2013-12-03)
Mir-29 microRNA families are involved in regulation of various types of cancers. Although Mir-29 was shown to play an inhibitory role in tumorigenesis, the role of Mir-29 in breast cancer still remains obscure. In this study, we showed that Mir-29a
In vitro and in vivo reversal of multidrug resistance in a human leukemia-resistant cell line by mdr1 antisense oligodeoxynucleotides.
C Cucco, B Calabretta
Cancer Research null
Delivery of c-myb antisense oligodeoxynucleotides to human neuroblastoma cells via disialoganglioside GD(2)-targeted immunoliposomes: antitumor effects.
G Pagnan, D D Stuart, F Pastorino, L Raffaghello, P G Montaldo, T M Allen, B Calabretta, M Ponzoni
Journal of the National Cancer Institute null
Resistance to apoptosis in CTLL-2 cells constitutively expressing c-Myb is associated with induction of BCL-2 expression and Myb-dependent regulation of bcl-2 promoter activity
Salomoni, P., et al
Proceedings of the National Academy of Sciences of the USA, 94, 3296-3301 (1997)
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