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05-1470

Sigma-Aldrich

Anti-Raptor Antibody, clone 1H6.2

clone 1H6.2, from mouse

Synonyme(s) :

P150 target of rapamycin (TOR)-scaffold protein, p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats, regulatory associated protein of mTOR

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

mouse

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

1H6.2, monoclonal

Espèces réactives

mouse, human

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG1κ

Numéro d'accès NCBI

NP_065812.1

Numéro d'accès UniProt

Q8N122

Conditions d'expédition

wet ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... RPTOR(57521)
mouse ... Rptor(74370)

Description générale

Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates.

Spécificité

This antibody recognizes Raptor.

Immunogène

Epitope: Unknown
Recombinant protein corresponding to human Raptor.

Application

Anti-Raptor Antibody, clone 1H6.2 detects level of Raptor & has been published & validated for use in WB, IP & IC.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Raptor in NIH/3T3 cells.

Immunoprecipitation Analysis: 20 µg from a previous lot immunoprecipitated Raptor in HEK293 cell lysate.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling

Qualité

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/ml of this antibody detected Raptor on 10 µg of HEK 293 cell lysate.

Description de la cible

~ 150 kDa

Forme physique

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Remarque sur l'analyse

Control
HEK 293 cell lysate

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Host mTORC1 signaling regulates andes virus replication.
McNulty, S; Flint, M; Nichol, ST; Spiropoulou, CF
Journal of virology null
Julia Dyachok et al.
The Journal of biological chemistry, 291(43), 22414-22426 (2016-09-03)
The mechanistic target of rapamycin complex 1 (mTORC1) coordinates cell growth with its nutritional, hormonal, energy, and stress status. Amino acids are critical regulators of mTORC1 that permit other inputs to mTORC1 activity. However, the roles of individual amino acids
Milagros Suarez et al.
Oncotarget, 12(10), 955-966 (2021-05-21)
The MAPK-interacting kinases 1 and 2 (MNK1/2) have generated increasing interest as therapeutic targets for acute myeloid leukemia (AML). We evaluated the therapeutic potential of the highly-selective MNK1/2 inhibitor Tomivosertib on AML cells. Tomivosertib was highly effective at blocking eIF4E
Jing Lu et al.
Blood, 122(9), 1610-1620 (2013-07-03)
Multiple myeloma (MM) is the second most common hematologic malignancy. Despite recent treatment advances, it remains incurable. Here, we report that Pim2 kinase expression is highly elevated in MM cells and demonstrate that it is required for MM cell proliferation.
Shan Ma et al.
Experimental eye research, 135, 1-13 (2015-04-19)
Cones are the primary photoreceptor (PR) cells responsible for vision in humans. They are metabolically highly active requiring phosphoinositide 3-kinase (PI3K) activity for long-term survival. One of the downstream targets of PI3K is the kinase mammalian target of rapamycin (mTOR)
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