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03-0191-00

Millipore

SMC® Human IFN-β1 High Sensitivity Immunoassay Kit

Synonyme(s) :

Interferon β-1 immunoassay kit, Interferon beta-1 Single Molecule Counting Kit, Interferon beta-1 immunoassay kit, Interferon beta-1 ultra-sensitive immunoassay

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Fabricant/nom de marque

SMC®

assay range

sensitivity: 0.15 pg/mL
(lower limit of quantification)
inter-assay cv: 13%
intra-assay cv: 6%

Technique(s)

ELISA: suitable
single molecule counting: suitable

Maladie(s) pertinente(s)

infectious diseases; autoimmune diseases; neurodegenerative disorders

Température de stockage

2-8°C

Description générale

A member of the type I interferon family of glycoprotein second messengers, human Interferon Beta (IFN-ß1) has been associated with antiviral activity as a secreted factor from infected cells that mediates innate immunity in neighboring cells. In addition, IFN-β1 has also been utilized as treatment for Multiple Sclerosis (MS) due to its ability to ameliorate inflammation in the central nervous system in this disease.

The SMC® Human IFN-β1 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

Spécificité

Cross Reactivity:
Cross-reactivity to the following analytes were tested with the following results:
  • human IFN-α2 – 0.2%
  • human IFN-γ – 0.3%
  • human IL-29 (IFN-λ1) – 0.2%
  • human IL-28A (IFN-λ2) – 0.2%
  • human IL-28B (IFN-λ3) – 0.3%
  • human IFN-ω – 0.2%

Accuracy:
  • Minimum of 5 serum samples spiked with average recovery of 88% with a range of 78-100%
  • Minimum of 5 plasma samples spiked with average recovery of 93% with a range of 75-113%

Application

Research Category:
  • Inflammation & Immunology
  • Immune Response
  • Infectious Diseases
  • Signaling
  • Neuroscience

Research Sub-Category:
  • Inflammatory Disease
  • Immune Response
  • Autoimmune disease
  • Neurodegenerative Diseases
  • Neuroregenerative Medicine
  • Virology
  • Infectious Diseases - Viral
  • Immunological Signaling

The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human IFN-β1 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IFN-β1 in human serum and plasma.

Caractéristiques et avantages

This new streamline kit has the following features including:

  • Improved options for sample prep and incubation
  • Lyophilization of the standard provides the kit at one storage condition
  • Increased reagent volumes to accommodate automation
  • Validated to run on both SMCxPRO® and Erenna

Conditionnement

96 well plate

Composants

  • Assay Buffer
  • IFN-β1 Coated Beads
  • Standard Diluent
  • IFN-β1 Detection Antibody
  • IFN-β1 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B

Informations légales

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictogrammes

Skull and crossbonesHealth hazard
GHS06,GHS08

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Contenu apparenté

Power of High Sensitivity Assays in Immunology Research | SMC® Technology

See how you can enhance your immunology research through the power of high sensitivity immunoassays by harnessing Single Molecule Counting (SMC®) technology to precisely characterize interferons, interleukins, and immunoglobins.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

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