906468
SLAM-VproS-13CHD2 Methyl Labeling Kit
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About This Item
Produits recommandés
Technique(s)
bio NMR: suitable
Niveau de qualité
Conditions d'expédition
dry ice
Température de stockage
−70°C
Catégories apparentées
Description générale
SLAM-VproS-13CHD2 kit has 13CHD2 isotopomer precursor and contains protocol instructions for creation of isotopically-labeled proteins.
Application
For solid-state NMR applications and dynamic studies.
For regio- and/or stereo- specific isotope labelilng of Isoleucine, Leucine, Valine, and Alanine with 13CHD2 isotopomers.
For regio- and/or stereo- specific isotope labelilng of Isoleucine, Leucine, Valine, and Alanine with 13CHD2 isotopomers.
SLAM-VproS-13CHD2 kit is used for stereospecific labeling of valine with 13CHD2 isotopomer. This kit has been tested with protein isotopic labeling in E. coli. It is beneficial for sample stability, which reduces sample heating because of high power decoupling when studying the side chain dynamics of proteins through solid-state NMR spectroscopy.
Conditionnement
This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.
Code de la classe de stockage
11 - Combustible Solids
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Current opinion in structural biology, 32, 113-122 (2015-04-17)
Nuclear magnetic resonance (NMR) spectroscopy is a uniquely powerful tool for studying the structure, dynamics and interactions of biomolecules at atomic resolution. In the past 15 years, the development of new isotopic labeling strategies has opened the possibility of exploiting
Current opinion in structural biology, 35, 60-67 (2015-09-26)
Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that
Articles
Methyl Isotope-Labeling of Proteins from NMR-Bio
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
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