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796-0477

HPLC Analysis of Cytochrome c Species Variants on Discovery® BIO PolyMA-SCX (Ion Exchange)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Discovery BIO PolyMA-SCX, 5 cm x 4.6 mm, 5 μm particles (59601-U)
mobile phase: [A] 20 mM Bis-Tris HCl, pH 7.0; [B] 20 mM Bis-Tris HCl, 0.5M NaCl, pH 7.0; 24 to 69% B in 20 min. (linear)
flow rate: 0.5 mL/min
column temp.: 25 °C
detector: UV, 280 nm
injection: 10 μg each variant

suitability

application for HPLC

application(s)

life science and biopharma

Analysis Note

This Figure shows the separation of three cytochrome c variants on Discovery BIO PolyMA-SCX. Note the difference in elution order compared to the reversed-phase separation on Discovery BIO Wide Pore C18 shown in product G001581.

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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