SRP0178

Histone H4 full length human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• Synonym(s): HIST2H4A
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥80% (SDS-PAGE)
• form: aqueous solution
• mol wt: 12.1 kDa
• packaging: pkg of 1 mg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_003548
• UniProt accession no.: P62805
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... HIST2H4A(8370)

Description

General description

Human Histone 4 (HIST2H4A) (GenBank Accession No. NM_003548), amino acids 2-104 (end) with N-terminal His-tag, MW = 12.1 kDa, expressed in an E. coli expression system.

Application

Suitable substrate for histone methyltransferases.

Biochem/physiol Actions

Histones H2B and H4 are found to become mobilized during an infection with HSV-1 (herpes simplex virus-1) and become available for binding to viral genome.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70°C. Note: Protein is very sensitive to freeze/thaw cycles.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable