SAB4200821
Anti-Fibroblast Surface Protein antibody, Mouse monoclonal
clone 1B10, purified from hybridoma cell culture
Synonym(s):
FSP
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
1B10, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μL
concentration
~ 1 mg/mL
technique(s)
immunofluorescence: 5-10 μg/mL using Human foreskin fibroblast Hs68 cells.
isotype
IgM
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... S100A4(6275)
General description
A surface protein characterizing human fibroblasts and fibroblast cell lines, as well as tissue macrophages and 95% of the peripheral blood monocytes. This fibroblast protein antigen has also been demonstrated on malignant fibrosarcoma tissue, human synovial, foreskin and thymic fibroblasts and it is absent from human epithelial cells and lymphocytes.
Specificity
Monoclonal Anti-Human Fibroblast Surface Protein specifically recognizes human fibroblast cells, tissue macrophages and peripheral monocytes .Due to its specific reaction against human fibroblasts, the antibody is useful for removal of fibroblasts from human cultured cells either by inhibition of fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement.
Immunogen
Cultured human thymic fibroblasts
Application
The antibody is recommended for use in various immunological techniques including Immunofluorescence, flow cytometry, immunohistochemistry and immunoblotting.
Biochem/physiol Actions
Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells.Characterization and identification of cells is important for studying functional significance, correlation of cultures with tissue origin, identification of the lineage to which the cells belongs to or identifying the precursor status of the cells.
Physical form
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
Storage and Stability
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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C N Abboud et al.
Blood, 68(6), 1196-1200 (1986-12-01)
The murine IgG2a monoclonal antibody 6-19 binds to a wide variety of nonhematopoietic cells including human marrow-derived stromal cells but does not bind to marrow or peripheral blood cells. We studied the effects of this antibody and rabbit complement on
K H Singer et al.
Human immunology, 13(3), 161-176 (1985-07-01)
Long-term in vitro cultures of human thymic tissue were established and phenotypically characterized using monoclonal reagents that define distinct components of the human thymic microenvironment. The epithelial component of the thymus, defined by monoclonal antibodies TE-3, TE-4, BBTECS, and AE1
N SundarRaj et al.
Current eye research, 3(4), 637-644 (1984-04-01)
Several monoclonal antibodies were developed to cell-surface antigens of human corneal fibroblasts grown in culture. Indirect immunohistochemical analysis showed that the antigens recognized by these monoclonal antibodies were also present on the skin fibroblasts. However, these antigens were not detectable
K H Singer et al.
The Journal of investigative dermatology, 92(2), 166-170 (1989-02-01)
A complement fixing IgM monoclonal antibody (1B10) that reacts with surface membrane molecules of human fibroblasts, tissue macrophages, and peripheral monocytes was produced. In Western blot analysis of detergent extracts of cultured human foreskin fibroblasts, antibody 1B10 detected protein bands
Lei Shangguan et al.
Stem cells (Dayton, Ohio), 30(12), 2810-2819 (2012-10-05)
Bone marrow mesenchymal stem cells (BM-MSCs) have multiple therapeutic potentials for regenerative, anti-inflammatory, and immunomodulatory purposes and also show promise as vehicles for gene therapy of various metastatic cancers based on their tumor-tropic capacity. However, BM-MSCs are also a source
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