SAB4200773
Anti-Pentraxin 3 antibody, Mouse monoclonal
clone PTX3-5, purified from hybridoma cell culture
Synonym(s):
Pentaxin-related protein PTX3, TNF alpha-induced protein 5, TSG-14, Tumor necrosis factor alpha-induced protein 5, Tumor necrosis factor-inducible gene 14 protein
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All Photos(3)
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
PTX3-5, monoclonal
form
buffered aqueous solution
mol wt
~47 kDa
species reactivity
human
concentration
~1 mg/mL
technique(s)
immunoblotting: 0.5-1 μg/mL using purified recombinant human Pentraxin 3 protein
immunoprecipitation (IP): 1-2 μg/test using lysate of HEK-293T cells over-expressing human Pentraxin 3
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PTX3(5806)
General description
Anti-Pentraxin 3 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the PTX3-5 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with recombinant human pentraxin 3 protein expressed in HEK-293 cells. Pentraxin 3 (PTX3), also called tumor necrosis factor inducible gene 14 protein (TSG-14), is a highly glycosylated secreted protein usually found as covalently-bound homooctamers. PTX3 belongs to the pentraxin subfamily, sharing the C-terminal domain with short pentraxins and contains a unique N-terminal domain. PTX3 is majorly present in monocytes, macrophages, fibroblasts, endothelial cells and adipocytes. It is an acute-phase protein mapped to human chromosome 3q25.32.
Specificity
Anti-Pentraxin 3 antibody, Mouse monoclonal recognizes both glycosylated and non-glycosylated human PTX3.
Immunogen
recombinant human Pentraxin 3 protein expressed in HEK-293 cells
Application
Anti-Pentraxin 3 antibody, Mouse monoclonal may be used in immunoblotting and immunoprecipitation.
Biochem/physiol Actions
Pentraxin 3 (PTX3) is released in response to primary inflammatory signals, such as toll-like receptor engagement, tumor necrosis factor α (TNFα) and interleukin 1β (IL-1β). It regulates innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility. PTX3 acts as a soluble pathogen recognition receptor and aids resistance against pathogens. It participates in the removal of apoptotic cells during an immune response. PTX3 associates with complement factor H and associates with synovial fluid in rheumatoid arthritis and also in conditions of sepsis. PTX3 is suppressed by pitavastatin, making it a biomarker for inflammatory cardiovascular disease.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Pentraxin 3: a novel biomarker for inflammatory cardiovascular disease
Inoue K, et al.
International Journal of Vascular Medicine, 2012 (2012)
Narae Hwang et al.
Korean journal of ophthalmology : KJO, 30(6), 468-478 (2016-12-17)
To investigate the production of long pentraxin 3 (PTX3) in response to tunicamycin-induced endoplasmic reticulum (ER) stress and its role in ER stress-associated cell death, PTX3 expression was evaluated in the human retinal pigment epithelial cell line, ARPE-19. PTX3 production
F Fazzini et al.
Arthritis and rheumatism, 44(12), 2841-2850 (2002-01-05)
To verify whether the prototypical long pentraxin PTX3 represents an indicator of the activity of small-vessel vasculitis. Concentrations of PTX3, a pentraxin induced in endothelium by cytokines, were measured by enzyme-linked immunosorbent assay in the sera of 43 patients with
B Muller et al.
Critical care medicine, 29(7), 1404-1407 (2001-07-11)
To evaluate the recently discovered long pentraxin PTX3 in plasma of critically ill patients and to compare it with the classic short pentraxin C-reactive protein and with other indicators of inflammation. A cohort study on plasma samples. Medical intensive care
Kenji Inoue et al.
International journal of vascular medicine, 2012, 657025-657025 (2012-02-22)
Numerous studies have recently examined the role of pentraxin 3 (PTX3) in clinical situations. The pentraxin family includes C-reactive protein (CRP); however, unlike CRP, PTX3 is expressed predominantly in atherosclerotic lesions that involve macrophages, neutrophils, dendritic cells, or smooth muscle
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