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SAB4200541

Sigma-Aldrich

Anti-ADAR1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-136 kDa double-stranded RNA-binding protein, Anti-DRADA, Anti-DSH, Anti-G1P1, Anti-IFI-4, Anti-IFI4, Anti-K88DSRBP, Anti-P136, Anti-adenosine deaminase acting on RNA 1-A, Anti-adenosine deaminase, RNA-specific, Anti-double-stranded RNA-specific adenosine deaminase, Anti-interferon-induced protein 4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~110 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 10-20 μg using lysates of human HepG2 or HEK-293T cells.
indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells.
western blot: 2-4 μg/mL using whole extracts of human HEK-293T cells.

UniProt accession no.

P55265

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... ADAR(505134)
human ... ADAR(103)
mouse ... Adar(56417)
rat ... Adar(81635)

Related Categories

General description

Adenosine deaminase that acts in RNA (ADAR) exists in two isoforms namely, ADAR1 and ADAR2, in mammals. Both the isoforms are expressed in variety of tissues but in general, the pre-mRNA being edited represents receptors of the central nervous systems. ADAR1 has several isoforms which include the full length ADAR1 (150 kDa) and two functionally active short isoforms (80 and 110 kDa).

Immunogen

synthetic peptide corresponding to an internal s of human ADAR1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, bovine, pig and monkey.

Application

Anti-ADAR1 antibody produced in rabbit has been used in
  • immunoblotting
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

Adenosine deaminase that acts in RNA 1 (ADAR1) play an important role in A-to-I RNA editing. In addition, it is also implicated in differentiating cells of embryo and adult tissues, facilitating cell survival and permitting their further differentiation and maturation. Its role in embryonic development is specifically observed within the hematopoietic lineage as well as in adult hematopoietic progenitor cells (HPCs). ADAR1 plays a key role in hematopoietic stem cell maintenance. It also block interferon signaling, that might help organisms to fight against the harmful effects of interferon activation linked with various diseases, such as chronic inflammation, autoimmune disorders and cancer.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
088M4783V
122M4772V

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Yael Nemlich et al.
Nature communications, 9(1), 2154-2154 (2018-06-02)
Melanoma cells use different migratory strategies to exit the primary tumor mass and invade surrounding and subsequently distant tissues. We reported previously that ADAR1 expression is downregulated in metastatic melanoma, thereby facilitating proliferation. Here we show that ADAR1 silencing enhances
Guermarie Velazquez-Torres et al.
Nature communications, 9(1), 461-461 (2018-02-02)
Previously we have reported that metastatic melanoma cell lines and tumor specimens have reduced expression of ADAR1 and consequently are impaired in their ability to perform A-to-I microRNA (miRNA) editing. The effects of A-to-I miRNAs editing on melanoma growth and
A-to-I miR-378a-3p editing can prevent melanoma progression via regulation of PARVA expression
Velazquez-Torres G, et al.
Nature Communications, 9(1), 461-461 (2018)
ADAR1 is required for hematopoietic progenitor cell survival via RNA editing
XuFeng R, et al.
Proceedings of the National Academy of Sciences of the USA, 106(42), 17763-17768 (2009)
ADAR1 is essential for the maintenance of hematopoiesis and suppression of interferon signaling
Hartner JC, et al.
Nature Immunology, 10(1), 109-109 (2009)
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