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SA20

Supelco

Starch (HK) Assay Kit

sufficient for 20 assays

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About This Item

UNSPSC Code:
12164500
NACRES:
NA.84

usage

sufficient for 20 assays

Quality Level

analyte chemical class(es)

sugars

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Application

This kit is for the quantitative, enzymatic determination of native starch in food and other materials using the amyloglucosidase-hexokinase-glucose 6-phosphate dehydrogenase enzymatic assay. The hydrolysis of starch to glucose is catalyzed by amyloglucosidase. Glucose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in a reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The increase in absorbance at 340 nm is directly proportional to the glucose concentration.

Kit Components Only

Product No.
Description

  • glucose assay reagent 20 mL

  • starch assay reagent 1 mL/vial

  • starch assay standard 5 g

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

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Fei-Fei Chu et al.
International journal of molecular sciences, 16(10), 23355-23368 (2015-10-02)
Polyphosphate (Poly-P) accumulation has been reported in Chlorella vulgaris under nitrogen deficiency conditions with sufficient P supply, and the process has been demonstrated to have great impact on lipid productivity. In this article, the utilization of polyphosphates and the regreening
Hakwon Yoon et al.
Nanomaterials (Basel, Switzerland), 9(11) (2019-11-02)
Nanoscale zerovalent iron (nZVI) is the most widely used nanomaterial for environmental remediation. The impacts of nZVI on terrestrial organisms have been recently reported, and in particular, plant growth was promoted by nZVI treatment in various concentrations. Therefore, it is
Xiao-Fei Shen et al.
Water research, 81, 294-300 (2015-06-18)
In this study the heterotrophic cultivation of Chlorella vulgaris NIES-227 fed with glucose was investigated systematically using six media types; combinations of nitrogen repletion/depletion and phosphorus repletion/limitation/depletion. It was found that a high yield of fatty acids (0.88 of fed
Beutler, H.
Methods of Enzymatic Analysis, 6, 2-10 (1984)
Amelia Torcello-Gómez et al.
Food chemistry, 272, 18-25 (2018-10-13)
An innovative procedure for plant chloroplasts isolation has been proposed, which consists of juice extraction by physical fractionation from plant material and recovery of its chloroplast-rich fraction (CRF) by centrifugation. This simple method has been applied to pea vine haulm

Articles

Nuclear magnetic resonance (NMR) spectroscopy measures the extent to which a glycan or other molecule distorts a magnetic field.

Protocols

Enzymatic methods for food analysis are highly specific and offer considerable time and cost savings over other methods, especially from the sample preparation standpoint. We offer a wide variety of convenient kits and reagents for rapid and reliable enzymatic food analysis.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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