S8140
SSPE Buffer
for Northern and Southern blotting, powder blend
Synonym(s):
Saline-Sodium Phosphate-EDTA Buffer
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About This Item
UNSPSC Code:
41105319
NACRES:
NA.25
Recommended Products
grade
for molecular biology
Quality Level
form
powder blend
pH
7.2-7.6 (20 × in water, high purity, ambient)
solubility
water, high purity: 20 ×, clear, colorless
foreign activity
DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected
storage temp.
room temp
Related Categories
General description
SSPE Buffer 20x powder is a standard reagent in Southern and Northern hybridization procedures. This powder should be reconstituted to the indicated volume with molecular biology grade water before use. The resulting 20x liquid can be used straight or diluted as needed.
Application
- Influence of Saline Buffers over the Stability of High-Annealed Gold Nanoparticles Formed on Coverslips for Biological and Chemosensing Applications.: This study explores the role of saline buffers in maintaining the stability of high-annealed gold nanoparticles used in biological and chemosensing applications. The findings highlight the potential for enhanced sensor performance in detecting biological molecules (Zhou & Ionescu, 2020).
- Quantification of Surface Etching by Common Buffers and Implications on the Accuracy of Label-Free Biological Assays.: This research assesses the impact of various buffers, including SSPE buffer, on the surface etching of substrates used in label-free biological assays. The results indicate that buffer-induced surface changes can significantly affect assay accuracy (Ahn et al., 2012).
Features and Benefits
- Easy to reconstitute powder
- Multi-purpose use in hybridizations
Components
Final SSPE Buffer 20x contains 0.02M EDTA and 2.98M NaCl in 0.2M phosphate buffer (pH 7.4).
related product
Product No.
Description
Pricing
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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New Biotechnology (2008)
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Igor V. Kutyavin
Nucleic Acids Research, 28(2) (2000)
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