S1073

Anti-STUB1/CHIP antibody, mouse monoclonal

clone ST21.55, purified from hybridoma cell culture

• Synonym(s): Anti-C terminus of HSC70-interacting protein (CHIP), Anti-HSPABP2, Anti-Heat shock protein A binding protein 2 (c-terminal), Anti-NY-CO-7, Anti-SDCCAG7, Anti-STIP1 homologous and box-containing protein 1 (STUB1), Anti-Serologically defined colon cancer antigen 7, Anti-UBOX1
• MDL number: MFCD02262541
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: ST21.55, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~35 kDa
• species reactivity: rat, human, bovine
• concentration: ~1.0 mg/mL
• technique(s): indirect ELISA: suitable; western blot: 2.5-5 μg/mL using HeLa total cell extract.
• isotype: IgG2b
• UniProt accession no.: Q9UNE7
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: bovine ... STUB1(504565) ; human ... STUB1(10273) ; mouse ... Stub1(56424) ; rat ... Stub1(287155)

Description

General description

Monoclonal Anti-STUB1/CHIP (mouse IgG2b isotype) is derived from the hybridoma ST21.55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 30-45 of human STUB1/CHIP. STUB1/CHIP (carboxyl terminus of Hsc70-interacting protein) consists of three functional domains: a tetratricopeptide repeat (TRP) at the amino terminus, a U- box domain at the C-terminus, and a highly charged region separating the two.

STUB1 (STIP1 homology and U-box containing protein 1) or CHIP (carboxyl terminus of Hsc70- interacting protein) is an E3 ubiquitin ligase, (2) that is located on human chromosome 16p13.3.

Immunogen

synthetic peptide corresponding to amino acids 30-45 of human STUB1/CHIP.

Application

Anti-STUB1/CHIP antibody, mouse monoclonal has been used in immunoblotting and enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

In lens epithelial cells, knocking down CHIP (carboxyl terminus of Hsc70- interacting protein) decreased the reaction of heat shock and potential of protein quality control. It plays a major role in neurodegeneration and also controls autophagic flux. It can deactivate NF-κB signaling and damage the capability of migration and invasion in gastric cancer cells.

STUB1/CHIP (carboxyl terminus of Hsc70-interacting protein) is such a cofactor, which interacts, among others, with Hsc70 and acts as a U-box-dependent E3 ubiquitin ligase. STUB1/CHIP can also act as a direct chaperone of p53, both under physiological and stress conditions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves