RAB0245
Mouse IL-10 ELISA Kit
for serum, plasma and cell culture supernatant
Synonym(s):
IL-10, Interleukin-10
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About This Item
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species reactivity
mouse
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
capture ELISA: suitable
input
sample type cell culture supernatant(s)
sample type serum
sample type plasma
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 45 pg/mL
standard curve range: 78.13-5000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Il10(16153)
General description
The Mouse IL-10 (Interleukin-10) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of IL-10 in serum, plasma, cell culture supernatants and urine.
Immunogen
Recombinant Mouse IL-10
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
IL-10 (interleukin 10) is an anti-inflammatory cytokine. In mouse, IL-10 produced by the CD4+ Th2 (T helper cell 2) subset, inhibits the expression of cytokines specifically IFN-3 (interferon), by Thl (T helper cell 1) clones. mIL-10 has pleiotropic property and it interacts with various cell types such as thymocytes, cytotoxic T cells, mast cells, B cells, and macrophages. Polymorphisms in the IL-10 gene are associated with higher risk of preeclampsia, ankylosing spondylitis, nasopharyngeal carcinoma and basal cell carcinoma. B- cell specific IL-10 might have a suppressive role in systemic lupus erythematosus (SLE).
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Kit Components Also Available Separately
Product No.
Description
SDS
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
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Clinical significance of IL-2 and IL-10 gene polymorphisms and serum levels in patients with basal-cell carcinoma.
Biomarkers in Medicine, 10(2), 185-195 (2016)
Interleukin 10(IL-10) inhibits cytokine synthesis by human monocytes: an autoregulatory role of IL-10 produced by monocytes.
The Journal of Experimental Medicine, 174(5), 1209-1220 (1991)
The Influence of Bone Marrow-Secreted IL-10 in a Mouse Model of Cerulein-Induced Pancreatic Fibrosis.
BioMed Research International (2016)
Investigations into the association between polymorphisms in the interleukin-10 gene and risk of early-onset preeclampsia.
Genetics and molecular research : GMR, 14(4), 19323-19328 (2015)
IL-10 Production Is Critical for Sustaining the Expansion of CD5+ B and NKT Cells and Restraining Autoantibody Production in Congenic Lupus-Prone Mice.
PLoS ONE, 11(3), e0150515-e0150515 (2016)
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