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R4880

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule) antibody produced in goat

affinity isolated antibody, lyophilized powder

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

technique(s)

immunoelectrophoresis: suitable

storage temp.

2-8°C

target post-translational modification

unmodified

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General description

Binds all rabbit Igs.

Application

Anti-Rabbit IgG (whole molecule) antibody produced in goat is suitable as secondary antibody in immunoblotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Other Notes

Antibody adsorbed with human serum proteins.

Physical form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Reconstitution

Reconstitute with 0.135 M sodium chloride.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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On-site quantification of human urinary albumin by a fluorescence immunoassay.
Sunga Choi et al.
Clinical chemistry, 50(6), 1052-1055 (2004-05-27)
M Rudling et al.
The American journal of pathology, 153(6), 1923-1935 (1998-12-10)
The high-affinity degradation of low-density lipoprotein (LDL) is enhanced 3- to 100-fold in leukemic blood cells from patients with acute myelogenous leukemia (AML), suggesting an increased cellular LDL receptor expression. There are, however, inconsistencies regarding the published properties of LDL
Jinsu Kim et al.
ACS applied materials & interfaces, 7(4), 2891-2898 (2015-01-13)
We demonstrated a lateral flow immunoassay (LFA) for detection of viruses using fluorescently labeled M13 bacteriophage as reporters and single-reporter counting as the readout. AviTag-biotinylated M13 phage were functionalized with antibodies using avidin-biotin conjugation and fluorescently labeled with AlexaFluor 555.
Shanshan Huang et al.
Pathology, research and practice, 215(4), 794-800 (2019-01-27)
Numbers of studies have indicated that miRNA-708 plays an important role in many types of cancer. However, the role of miRNA-708 in pancreatic ductal adenocarcinoma (PDAC) has yet to be fully elucidated. The present study aimed to investigate the role
Peter Engelhardt et al.
Methods in molecular biology (Clifton, N.J.), 369, 387-405 (2007-07-28)
Standard immunogold-labeling methods in transmission electron microscopy (TEM) are unable to locate immunogold particles in the depth direction. This inability does not only concern bulky whole mounts, but also sections. A partial solution to the problem is stereo inspection. However

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