L8262

Lectin from Ulex europaeus (gorse, furze)

biotin conjugate, lyophilized powder

• Synonym(s): Ulex europaeus agglutinin, UEA-I
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• conjugate: biotin conjugate
• Quality Level: 200
• form: lyophilized powder
• potency: <50 μg/mL agglutination activity
• composition: Protein, ~85% E1%/280
• extent of labeling: ~3 mol biotin per mol protein
• storage temp.: 2-8°C

Description

Application

Lectin from Ulex europaeus (gorse, furze) has been used:

• as a Paneth cells marker in immunohistochemistry at 1:1,000 dilution
• in double staining for endothelial cells for quantitation of blood vessels
• in histochemical staining of bronchial epithelial cells

Biochem/physiol Actions

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Packaging

Package size based on protein content.

Linkage

Conjugates are prepared from affinity purified lectin.

Physical form

Contains sodium citrate

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves