L4516

Lipopolysaccharides from Escherichia coli O127:B8

BioXtra, suitable for cell culture, γ-irradiated

• Synonym(s): LPS
• EC Number: 297-473-0
• MDL number: MFCD00212998
• UNSPSC Code: 12352211
• NACRES: NA.75

Properties

• biological source: Escherichia coli (O127:B8)
• Quality Level: 200
• sterility: γ-irradiated
• product line: BioXtra
• form: lyophilized powder
• purified by: gel-filtration chromatography
• technique(s): cell culture | mammalian: suitable
• impurities: <1% Protein (Lowry)
• solubility: H₂O: 5 mg/mL, slightly hazy
• storage temp.: 2-8°C

Description

Application

Lipopolysaccharides from Escherichia coli 0127:B8 has been used:

• to study the LPS-induced macrophage survival in mice
• to elicit the secretion of cytokines by cattle PBMC
• to study apoptosis of highly susceptible macrophage cells in a LPS-induced proinflammatory environment
• to perform autophagy assay in BV2 and BV2-LC3 cells
• for stimulation of human monocytes, macrophages and mouse microglia cells
• to induce pentraxin-3 expression in 293T cells

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4). Being an immunogenic antigen, it enhances immune responses to soluble antigens.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Safety Information

• Pictograms: GHS06
• Signal Word: Danger
• Hazard Statements: H300
• Precautionary Statements: P264 - P270 - P301 + P310 - P405 - P501
• Hazard Classifications: Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable