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COO/COA

L-Leucine-7-amido-4-methylcoumarin hydrochloride

Name: <SC>L</SC>-Leucine-7-amido-4-methylcoumarin hydrochloride
Brand: SIGMA

fluorogenic, ≥98% (TLC), powder

Synonym(s):

H-L-Leu-AMC HCl

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About This Item

Empirical Formula (Hill Notation):

C₁₆H₂₀N₂O₃ · HCl

CAS Number:

62480-44-8

Molecular Weight:

324.80

MDL number:

MFCD00043262

UNSPSC Code:

12352204

PubChem Substance ID:

24896303

NACRES:

NA.32

product name

L-Leucine-7-amido-4-methylcoumarin hydrochloride,

Assay

≥98% (TLC)

form

powder

solubility

methanol: 50 mg/mL, clear, colorless to faintly yellow

fluorescence

λ_ex 327 nm; λ_em 349 nm (pH 8.0)
λ_ex 380 nm; λ_em 440 nm (Reaction product)

storage temp.

−20°C

SMILES string

Cl.CC(C)C[C@H](N)C(=O)Nc1ccc2C(C)=CC(=O)Oc2c1

InChI

1S/C16H20N2O3.ClH/c1-9(2)6-13(17)16(20)18-11-4-5-12-10(3)7-15(19)21-14(12)8-11;/h4-5,7-9,13H,6,17H2,1-3H3,(H,18,20);1H/t13-;/m0./s1

InChI key

VCRXITKKWBOQRZ-ZOWNYOTGSA-N

General description

L-Leucine-7-amido-4-methylcoumarin hydrochloride (Leu-AMC) is a fluorogenic peptidyl substrate for leucine aminopeptidase, an extracellular enzyme found abundantly in natural aquatic systems. This enzyme is one of the various enzymes used by bacteria for protein hydrolysis.

Application

L-Leucine-7-amido-4-methylcoumarin hydrochloride (Leu-AMC) as a substrate analog to determine leucine aminopeptidase activity

L-Leucine-7-amido-4-methylcoumarin hydrochloride has been used:

as a substrate in fluorescence-based soil assays
to determine leucine aminopeptidase activity of both Plasmodium falciparum M1 (PfA-M1) and PfA-M17 enzymes

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M17 leucine aminopeptidase of the human malaria parasite Plasmodium vivax

Lee JY, et al.

Molecular and Biochemical Parasitology, 170, 45-48 (2010)

A pilot experiment to assess the efficiency of pharmaceutical plant wastewater treatment and the decreasing effluent toxicity to periphytic biofilms.

Vincent Tardy et al.

Journal of hazardous materials, 411, 125121-125121 (2021-04-17)

Pharmaceutical industry effluents are complex and highly variable in time. Assessing the efficiency of a pharmaceutical industry wastewater treatment plant (WWTP) and the resulting decrease in effluent toxicity and ecological risk is thus not straightforward. We set up an original

Reconciling apparent variability in effects of biochar amendment on soil enzyme activities by assay optimization

Bailey VL, et al.

Soil Biology and Biochemistry, 43(2), 296-301 (2011)

Activity profiling of ectomycorrhiza communities in two forest soils using multiple enzymatic tests

Courty PE, et al.

The New phytologist, 167, 309-319 (2005)