Skip to Content
Merck
All Photos(3)

Key Documents

GE28-9365-41

HiPrep™ DEAE Fast Flow 16/10

Cytiva 28-9365-41

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
47101511
NACRES:
NA.56

ligand

diethylaminoethyl

description

Ion Exchanger Type (value)

packaging

pkg of 20 mL

manufacturer/tradename

Cytiva 28-9365-41

parameter

1.5 bar (22 psi) (Over the Packed Bed During Operation)
2 ml/min - 10 ml/min flow rate
22 psi

bed size

16 mm × 100 mm

bed volume

20 mL

column I.D.

16 mm

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning

2-14

working range

2-12

suitability

suitable for bioprocess medium

General description

HiPrep™ DEAE FF 16/10 is a weak anion exchanger prepacked with DEAE Sepharose Fast Flow, ready-to-use for fast, preparative separations of proteins and other biomolecules using ion exchange (IEX) chromatography.

HiPrep™ DEAE FF 16/10 is a prepacked, ready to use column for ion exchange chromatography. It provide sfast, preparative separations of proteins and other biomolecules.

Features and Benefits

  • 20 mL HiPrep™ columns provide fast, reliable, and reproducible preparative ion exchange separations.
  • Good flow rates and high capacity.
  • The industry standard for ion exchange chromatography.
  • Compatible with single-pump configurations and chromatography systems such as AKTA design.
  • Use a weak ion exchanger if the selectivity of a strong ion exchanger is insufficient.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Legal Information

HiPrep is a trademark of Cytiva

related product

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Purification of Membrane Proteins

This page shows how to perform a purification of His-tagged membrane proteins.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Ion Exchange Chromatography Troubleshooting

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

See All

Protocols

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service