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GE17-0529-01

Calmodulin-Sepharose™ 4B

Cytiva 17-0529-01, pack of 10 mL

Synonym(s):

Calmodulin Affinity Resin, Calmodulin-Sepharose

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

calmodulin

packaging

pack of 10 mL

manufacturer/tradename

Cytiva 17-0529-01

storage condition

(20% Ehtanol)

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

4-9

working range

4-9

storage temp.

2-8°C

General description

Calmodulin Sepharose™ 4B affinity media for purification of proteins with affinity for calmodulin, a highly conserved regulatory protein involved in many cellular processes in eukaryotic cells.

Calmodulin is a highly conserved regulatory protein found in all eukaryotic cells.This protein is involved in many cellular processes such as glycogen metabolism,cytoskeletal control, neurotransmission, phosphate activity and control of NAD?/NADP? Calmodulin binds proteins principally through their interactions with hydrophobic sites on its surface. These sites are exposed after a conformational change induced by the action of Ca2+ on separate Ca2+-binding sites. The binding of enzymes may be enhanced if the enzyme substrate is present and enzyme-substratecalmoldulin-Ca2+ complexes are particularly stable.

Features and Benefits

  • For single-step purification of native calmodulin-binding proteins
  • Suitable for tandem affinity purification (TAP) of protein complexes
  • Purification of calmodulin-regulated proteins from all eukaryotic cells

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mahmoud Hajj Chehade et al.
Cell chemical biology, 26(4), 482-492 (2019-01-29)
Ubiquinone (UQ) is a polyprenylated lipid that is conserved from bacteria to humans and is crucial to cellular respiration. How the cell orchestrates the efficient synthesis of UQ, which involves the modification of extremely hydrophobic substrates by multiple sequential enzymes

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This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from GE Healthcare.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

Performing a Purification with Calmodulin Sepharose 4B

This page shows how to purify ATPases, adenylate cyclases, protein kinases, phosphodiesterases and neurotransmitters by affinity chromatography using Calmodulin Sepharose 4B from GE Healthcare.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

Column Packing and Preparation Protocol for Affinity Chromatography

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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