G7663
Gelatin blocking buffer
for Western blotting, powder blend
Synonym(s):
blocking buffer
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About This Item
UNSPSC Code:
41105300
NACRES:
NA.25
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General description
Gelatin Blocking Buffer is a standard reagent in Western blotting procedures. It is used to block non-specific binding on the membrane, when using nylon or nitrocellulose (but not PVDF).
Application
Suitable for use as a non-specific blocking agent for Western blots.
Reconstitution
Dissolve entire contents of the bottle in a final volume of 1L molecular biology grade water. The resulting solution will be faint yellow in color and does not require filtering or heating before use.
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Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
In order to specifically detect an antigen or target molecule immobilized on a solid support, unoccupied binding sites on the support must be blocked against binding by probe and detection molecules.
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