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F4759

Sigma-Aldrich

Fibronectin bovine plasma

powder, BioReagent, suitable for cell culture

Synonym(s):

Fibronectin

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About This Item

EC Number:
289-149-2
MDL number:
MFCD00131062
UNSPSC Code:
12352202
eCl@ss:
42020141
NACRES:
NA.75

biological source

bovine plasma

Quality Level

200

sterility

sterile

product line

BioReagent

form

powder

mol wt

~450 kDa

packaging

pkg of 1 mg
pkg of 2 mg
pkg of 5 mg

concentration

≥1 mg protein/mL

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm2

solubility

H2O: 1 mg/mL at 37 °C (Store reconstituted solution in working aliquots at -20°C or lower.)

NCBI accession no.

NM_001163778.1

UniProt accession no.

P07589

shipped in

dry ice

storage temp.

−20°C

Gene Information

bovine ... FN1(2807940)

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General description

Fibronectin is a glycoprotein with multiple domains. It comprises a N-terminal, central binding domain and a heparin-binding domain. Bovine plasma fibronectin has polypeptide chains with disulfide linkage and corresponds to a molecular weight of 220 and 215 kDa.
Fibronectins are adhesive glycoproteins and are found both as cell surface proteins and in plasma. Fibronectin (FN) is a multifunctional glycoprotein, the gene of which is localized to human chromosome 2q34-36. This gene spans 75kb and contains 50 exons. It exists as two major isoforms, one soluble and one insoluble form. The former is present in plasma, whereas the insoluble form resides in tissues and extracellular matrix (ECM) of cartilage. There are around 20 different forms of FN due to alternative splicing of a variable region and two type III exons called Extra Domains A and B.

Application

Fibronectin from bovine plasma is used for the following applications:
  • Used for coating the glass coverslips for the culture of decidual stromal cells (DEC)
  • Cell movement assays (culture plates were coated with fibronectin)
  • Cell spreading and immunofluorescence
  • Protein specificity
  • Integration of sensor films into microfluidic chips
  • Histological and Immunohistochemical (IHC) analyses
  • Used in primary cell cultures from patients with lung carcinoma

Biochem/physiol Actions

Fibronectin is a regulator of protein scaffolding. It coordinates cellular processes like platelet functions, wound-healing, adhesion, migration and aggregation.Study shows that human dental pulp (HDP) cells rely on fibronectin (FN) for initial attachment and subsequent spreading. FN may exhibit mitogenic activity, promoting cell proliferation, and also play a role in regulating the differentiation process of pulp cells into odontoblasts. The presence of FN appears to influence the fate and development of HDP cells, specifically in their differentiation into odontoblasts, which are responsible for dentin formation in teeth.

Caution

As supplied, this product should be stored at -20°C and will remain active for at least 2 years.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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R W Redline et al.
The Journal of clinical investigation, 85(6), 1951-1958 (1990-06-01)
Because of their paternal antigens, the fetus and placenta may be considered an allograft in the maternal host. Local properties of the maternal-fetal interface, the placenta and decidua basalis, are important in preventing maternal immunologic rejection of the fetoplacental allograft.
Cigdem Aydin et al.
Human gene therapy, 18(1), 39-50 (2006-12-26)
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) selectively induces apoptosis in cancer cells but not in normal cells. However, studies have indicated that more than half of human tumors exhibit TRAIL resistance. Although the mechanism of TRAIL resistance is not
Toru Yoshihara et al.
PLoS genetics, 14(8), e1007545-e1007545 (2018-08-17)
It is uncertain which β4-galactosyltransferase (β4GalT; gene name, B4galt), β4GalT-5 and/or β4GalT-6, is responsible for the production of lactosylceramide (LacCer) synthase, which functions in the initial step of ganglioside biosynthesis. Here, we generated conditional B4galt5 knockout (B4galt5 cKO) mice, using
Wagner Shin Nishitani et al.
PloS one, 10(5), e0126440-e0126440 (2015-05-07)
A cell mechanical stimulation equipment, based on cell substrate deformation, and a more sensitive method for measuring adhesion of cells were developed. A probe, precisely positioned close to the cell, was capable of a vertical localized mechanical stimulation with a
Kristina Woodruff et al.
Scientific reports, 6, 23937-23937 (2016-04-01)
Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent transfections at up to 99% efficiency. The chip
View All Related Papers

Articles

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Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocols

Fibronectin Coating Protocol

Dilute fibronectin to the desired concentration. Optimum conditions for attachment are dependent on cell type and application. The typical coating concentration is 1 – 5 ug/cm2.Fibronectin coating protocol, products, and FAQs.

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