CWDS-IF647
ColorWheel® Antibody-Ready iFluor 647 Dye
for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)
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About This Item
UNSPSC Code:
41116015
NACRES:
NA.32
Recommended Products
product line
ColorWheel®
Quality Level
form
lyophilized
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
flow cytometry: suitable
fluorescence
λex 656 nm; λem 670 nm
compatibility
for use with ColorWheel® Dye-ready Antibodies (Required, sold separately)
greener alternative category
shipped in
ambient
storage temp.
2-8°C
General description
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
iFluor 647 succinimidyl ester is bright, stable far-red fluorescent dye that has spectral properties comparable to Alexa Fluor™ 647 and Cy dye. It exhibits an excitation max at 656 nm and an emission max at 670 nm with an extinction coefficient of 250,000 cm-1 M-1. It has stronger fluorescence and high photostability. iFluor 647 can be used for covalent labeling of antibodies and detection by flow cytometry. Its fluorescence is independent of pH from 3 to 11. It exhibits good stability, selectivity, and reactivity with protein amine groups.
iFluor 647 succinimidyl ester is bright, stable far-red fluorescent dye that has spectral properties comparable to Alexa Fluor™ 647 and Cy dye. It exhibits an excitation max at 656 nm and an emission max at 670 nm with an extinction coefficient of 250,000 cm-1 M-1. It has stronger fluorescence and high photostability. iFluor 647 can be used for covalent labeling of antibodies and detection by flow cytometry. Its fluorescence is independent of pH from 3 to 11. It exhibits good stability, selectivity, and reactivity with protein amine groups.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Application
Flow Cytometry: Excitation: 656 nm, Emission: 670 nm
Compatibility
Requires ColorWheel® Antibody (Sold Separately)
Physical form
CWDS-IF647-25UL Set Components
CWD-IF647-25UL - Colorwheel® Antibody-Ready iFluor 647 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL - Colorwheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
CWD-IF647-25UL - Colorwheel® Antibody-Ready iFluor 647 Dye
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
CWD-FINISH-25UL - Colorwheel® Finishing Buffer
Lyophilized from PBS. Contains no biocide or preservatives, such as azide.
Reconstitution
Reconstitute each vial by adding 25 μL PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
related product
Storage Class Code
11 - Combustible Solids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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