SK LU 1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93120835, human lung, Epithelial

• UNSPSC Code: 41106514

Properties

• biological source: human lung
• packaging: tube of 5 μg 93120835-DNA-5UG; pkg of vial of cells 93120835-1VL
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian lung adenocarcinoma

Cell Line Description

Derived from a 64 year old Caucasian female with lung adenocarcinoma (grade III). The line is tumourigenic in immunotolerant rats. HLA = Aw24, w32, w41, B27

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 10
D16S539: 8
D5S818: 11
D7S820: 9
THO1: 7
TPOX: 8,10
vWA: 16,17

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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