AR42J

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 93100618, rat pancreas, Pancreas cells

• UNSPSC Code: 41106514

Properties

• biological source: rat pancreas
• packaging: tube of 5 μg 93100618-DNA-5UG; pkg of vial of cells 93100618-1VL
• growth mode: Semi-adherent aggregates
• karyotype: Not specified
• morphology: Pancreas cells
• products: Amylase and exocrine enzymes
• receptors: Gastrin/CCKB, Epidermal growth factor (EGF), Somatostatin
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rat exocrine pancreatic tumour

Cell Line Description

Derived from a transplantable tumour of a rat exocrine pancreas. The line is tumourigenic in nude mice, and shows significant secretion of amylase and other exocrine enzymes.

Application

AR42J has been used to obtain a tumor mouse model by inoculating it subcutaneously in a female, athymic nude mouse. It has also been used to perform imaging studies.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Cells grow in hollow spheroid colonies that can attach loosely. Split cultures 1:2 every 48 hours into fresh flasks, maintain cultures between 1-9x100,000 cells/ml. Adherent cells should be dislodged using 0.2% EDTA. N.B. High cell viability cannot be ex

Other Notes

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