HP 6054

Reactivity: Anti-λ-Light chain, human, 93092704

• UNSPSC Code: 41106514

Properties

• biological source: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Myeloma: Sp2/0 Ag-14
• Reactivity: Anti-λ-Light chain, human
• technique(s): cell culture | mammalian: suitable
• isotype: IgG2a
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse Sp2/0 Ag-14 x Mouse BALB/c spleen

Cell Line Description

The antibody is directed against human kappa light chains.

Immunogen

Purified human immunoglobulin myeloma protein covalently coupled to polyaminostyrene beads

Application

Classification of human imunoglobulin

Culture Medium

DMEM + 2mM glutamine + 10mM HEPES + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 100,000 - 1,000,000 cells/ml; 5% CO₂; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.